|
| Status |
Public on Mar 04, 2016 |
| Title |
Investigation of small RNA and RNA-seq transcripts from soybean (Glycine max) DCL1 mutants |
| Organism |
Glycine max |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
In plants, DICER-LIKE1 is a critical enzyme in the biogenesis pathway of plant miRNAs. Zinc-finger nucleases were used to generate single and double-mutants of soybean DCL1 homologs. We created several small RNA libraries to investigate the effect on miRNA abundance in these various mutants. RNAseq transcript data was also generated in order to investigate targets of those impacted miRNAs.
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| |
|
| Overall design |
Genome-wide small RNA profiling was done by Illumina TruSeq sample preparation followed by high-throughput sequencing with Illumina HiSeq 2000 platform.
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| |
|
| Contributor(s) |
Meyers B, Curtin S, Hammond R |
| Citation(s) |
26681515 |
| |
| Submission date |
Dec 15, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Blake C. Meyers |
| E-mail(s) |
bmeyers@danforthcenter.org
|
| Phone |
314-587-1422
|
| Organization name |
Donald Danforth Plant Science Center
|
| Lab |
Meyers lab
|
| Street address |
975 N Warson Road
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63132 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL15008 |
Illumina HiSeq 2000 (Glycine max) |
|
| Samples (14)
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|
| Relations |
| BioProject |
PRJNA306049 |
| SRA |
SRP067410 |
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