|
| Status |
Public on Mar 04, 2016 |
| Title |
Soybean leaf WT rep2 |
| Sample type |
SRA |
| |
|
| Source name |
Soybean leaf
|
| Organism |
Glycine max |
| Characteristics |
tissue: Soybean leaf
genotype/variation: wildtype
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Materials were collected and flash frozen in liquid nitrogen. Total RNA was extracted by Tri reagent (Molecular Research Center, Inc.) according to manufacturer's protocol.
Small RNA libraries were constructed using the Illumina TruSeq Small RNA sample preparation kit RS-200-0012 and RNA-Seq libraries were prepared using the Illumina TruSeq RNA sample preparation kit RS-122-2001.
|
| |
|
| Library strategy |
ncRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
Soybean leaf, wildtype rep2
|
| Data processing |
For data processing, the adapter sequences were trimmed from raw sequence reads. The small RNA sequences were further trimmed to the lengths between 18 and 34 nucleotides. RNA-seq sequences were maintained at the length after adapter trimming. RNA-seq sequences were maintained at the length after adapter trimming.
Sequence data processing was conducted using in-house Perl scripts developed by Meyers lab in University of Delaware
Genome_build: JGI glyma v2
Supplementary_files_format_and_content: tag count file
|
| |
|
| Submission date |
Dec 15, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Blake C. Meyers |
| E-mail(s) |
bmeyers@danforthcenter.org
|
| Phone |
314-587-1422
|
| Organization name |
Donald Danforth Plant Science Center
|
| Lab |
Meyers lab
|
| Street address |
975 N Warson Road
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63132 |
| Country |
USA |
| |
|
| Platform ID |
GPL15008 |
| Series (1) |
| GSE76036 |
Investigation of small RNA and RNA-seq transcripts from soybean (Glycine max) DCL1 mutants |
|
| Relations |
| BioSample |
SAMN04338526 |
| SRA |
SRX1483313 |
