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Series GSE76491 Query DataSets for GSE76491
Status Public on Sep 09, 2019
Title Coordination of neuron production in mouse and human cerebral cortex by the homolog of Drosophila Mastermind protein
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The coordination of progenitor self-renewal, neuronal production and migration is essential to the normal development and evolution of the cerebral cortex. Numerous studies have shown that the Notch, Wnt/beta-catenin and Neurogenin pathways contribute separately to progenitor expansion, neurogenesis, and neuronal migration, but it is unknown how these signals are coordinated. In vitro studies suggested that the mastermind-like 1 (MAML1) gene, homologue of the Drosophila Mastermind, plays a role in coordinating the afore-mentioned signaling pathways, yet its role during cortical development remains largely unknown. Here we show that ectopic expression of dominant-negative MAML (dnMAML) causes exuberant neuronal production in the mouse cortex without disrupting neuronal migration. Comparing the transcriptional consequences of dnMAML and Neurog2 ectopic expression revealed a complex genetic network controlling the balance of progenitor expansion versus neuronal production. Manilpulation of MAML and Neurog2 in cultured human cerebral stem cells exposed interactions with the same set of signaling pathways. Thus, our data suggest that evolutionary changes that affect the timing, tempo and density of successive neuronal layers of the small lissencephalic rodent and large convoluted primate cerebral cortex depend on similar molecular mechanisms that act from the earliest developmental stages.
Overall design RNAseq after disruption of Notch and Neurognin2 signaling in human fetal glia in vitro and after in utero electroporation in the mouse dorsal cortex. Tissue was laser-microdissected. Cells propagated in vitro were magnetically seperated.
Contributor(s) Ayoub AE, Rakic P
Citation(s) 31416089
Submission date Jan 04, 2016
Last update date Sep 11, 2019
Contact name Albert E. Ayoub
Organization name Yale University
Department Neurobiology
Street address 333 Cedar St. SHM C317
City New Haven
State/province CT
ZIP/Postal code 06460
Country USA
Platforms (2)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (37)
GSM2026544 E11 replicate 1
GSM2026545 E11 replicate 2
GSM2026546 E12 replicate 1
BioProject PRJNA307569
SRA SRP068019

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Supplementary file Size Download File type/resource
GSE76491_RAW.tar 6.3 Mb (http)(custom) TAR (of RPKM)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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