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| Status |
Public on Jan 25, 2016 |
| Title |
Expression data from a cell model of Huntington disease |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Huntington disease (HD) is associated with increased nuclear accumulation of the repressor element-1 silencing transcription factor (REST) which govens a huge gene network. An alternative REST splicing event (∆E3) eliminates a motif essential for nuclear targeting of REST.
We used Affymetrix’s GeneChip® Mouse Gene 2.0 ST Array to compare global transcription between STHdhQ111/Q111 cells (a cell model of HD) treated with specific antisense oligos (ASOs) inducing ∆E3 and a control oligo, with a comparison to normal STHdhQ7/Q7 cells.
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| Overall design |
STHdhQ111/111 cells were treated with specific ASOs (inducing ∆E3) or a control oligo, and STHdhQ7/Q7 cells treated with a control oligo was used for a normal control. 3 representative total RNA samples (one for each group) of high quality were subject to transcriptional profiling.
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| Contributor(s) |
Chen G, Miller GM |
| Citation(s) |
28524599 |
| |
| Submission date |
Jan 25, 2016 |
| Last update date |
Feb 21, 2018 |
| Contact name |
Guo-Lin Chen |
| E-mail(s) |
gu.chen@neu.edu
|
| Phone |
6173736301
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| Organization name |
Northeastern University
|
| Street address |
360 Huntington Avenue
|
| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA309685 |
