|
Status |
Public on Oct 31, 2007 |
Title |
Ovarian Transcript Expression in Newborn Mouse |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
|
Summary |
Microarray analysis of normal newborn ovarian transcript levels, for use in comparison to array based studies of differential expression in mouse knockout models. Keywords: Genetic Modification
|
|
|
Overall design |
Newborn ovaries were pooled separately from wild type animals and total RNA isolated using RNeasy mini kit (Qiagen, CA). Newborn ovaries were collected within 12 hours of delivery. Animal experimentation was approved by the Institutional Animal Care and Use Committee of Baylor College of Medicine. Three independently pooled RNA samples from wild type used to generate biotinylated cRNA. Biotinylated cRNA was hybridized to GeneChip Mouse Expression Set 430 2.0 (Affymetrix, Inc.). Since three independent experiments were performed from three independent pools of wild type RNA, signal intensities for particular genes were averaged between the three chips.
|
|
|
Contributor(s) |
Rajkovic A, Choi Y, Qin Y, Ballow D, Pangas S |
Citation(s) |
18509161 |
|
Submission date |
May 10, 2007 |
Last update date |
Feb 11, 2019 |
Contact name |
Aleksandar Rajkovic |
E-mail(s) |
rajkovic@bcm.edu
|
Organization name |
Baylor College of Medicine
|
Street address |
One Baylor Plaza
|
City |
Houston |
State/province |
TX |
ZIP/Postal code |
77030 |
Country |
USA |
|
|
Platforms (3) |
GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
GPL340 |
[MOE430B] Affymetrix Mouse Expression 430B Array |
GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
Samples (4)
|
|
This SubSeries is part of SuperSeries: |
GSE11897 |
Lim Homeobox Gene, LHX8, Is Essential for Mouse Oocyte Differentiation and Survival |
|
Relations |
BioProject |
PRJNA109021 |