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| Status |
Public on Dec 31, 2016 |
| Title |
PfAP2-I-GFP-msp5MUT vs PfAP2-I-GFP 3D7 [timecourse 2] |
| Organism |
Plasmodium falciparum |
| Experiment type |
Expression profiling by array
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| Summary |
Obligate intracellular parasites must efficiently invade host cells in order to mature and be transmitted. For the malaria parasite Plasmodium falciparum, invasion of host red blood cells (RBCs) is essential. Here we describe a parasite-specific transcription factor belonging to the Apicomplexan Apetala 2 (ApiAP2) family that is responsible for regulating the expression of a subset of merozoite genes involved in RBC invasion (PfAP2-I). Our genome-wide analysis by ChIP-seq shows that PfAP2-I interacts with a specific DNA motif in the promoters of these genes. msp5 transcription levels decrease when the PfAP2-I DNA-binding motif is mutated in PfAP2-I-GFP parasites, showing that PfAP2-I must bind the DNA motif in order for msp5 to be transcribed.
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| Overall design |
Plasmodium falciparum strains AP2-I-GFP and AP2-I-GFP were highly synchronized and two parallel timecourses resulting in a total of 8 samples (4 wildtype PfAP2-I-GFP, 4 msp5 mutant) were hybridized against a Cy3-labeled reference pool of 3D7 mixed stage parasites on a two-color array. Starting at 18 hours post-invasion, samples were harvested in Trizol for total RNA extraction every 6 hours until 36h post-invasion and then every 3h until the end of the IDC (48h post-invasion).
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| Contributor(s) |
Santos JM, Ross P, Joshi P, Altenhofen LM, Campbell T, Schieler A, Cristea IM, Llinás M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Feb 10, 2016 |
| Last update date |
Jan 24, 2019 |
| Contact name |
Manuel Llinas |
| E-mail(s) |
manuel@psu.edu
|
| Phone |
8148673444
|
| Organization name |
Penn State University
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| Department |
Biochemistry and Molecular Biology
|
| Lab |
Manuel Llinas Lab
|
| Street address |
Millennium Science Complex, Pollock Rd
|
| City |
University Park |
| State/province |
Pennsylvania |
| ZIP/Postal code |
16802 |
| Country |
USA |
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| Platforms (1) |
| GPL15130 |
Agilent-037237 P.falciparum_Pf3D7v7.1_Transcriptome_apimito |
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| Samples (16)
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| GSM2059937 |
PfAP2-I-GFP 36 hours post invasion_2 |
| GSM2059938 |
PfAP2-I-GFP 39 hours post invasion_2 |
| GSM2059939 |
PfAP2-I-GFP 42 hours post invasion_2 |
| GSM2059940 |
PfAP2-I-GFP 45 hours post invasion_2 |
| GSM2059941 |
PfAP2-I-GFP 48 hours post invasion_2 |
| GSM2059942 |
PfAP2-I-GFP-msp5mut 18 hours post invasion_2 |
| GSM2059943 |
PfAP2-I-GFP-msp5mut 24 hours post invasion_2 |
| GSM2059944 |
PfAP2-I-GFP-msp5mut 30 hours post invasion_2 |
| GSM2059945 |
PfAP2-I-GFP-msp5mut 36 hours post invasion_2 |
| GSM2059946 |
PfAP2-I-GFP-msp5mut 39 hours post invasion_2 |
| GSM2059947 |
PfAP2-I-GFP-msp5mut 42 hours post invasion_2 |
| GSM2059948 |
PfAP2-I-GFP-msp5mut 45 hours post invasion_2 |
| GSM2059949 |
PfAP2-I-GFP-msp5mut 48 hours post invasion_2 |
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| This SubSeries is part of SuperSeries: |
| GSE77810 |
PfAP2-I-GFP-msp5MUT vs PfAP2-I-GFP 3D7 |
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| Relations |
| BioProject |
PRJNA311599 |
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