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| Status |
Public on Feb 12, 2016 |
| Title |
Gene expression change of M1 and BY-P41K-RPB7 in YPAD (OD600 reached 1) and after 12h Very High Gravity (VHG) fermentation |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
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| Summary |
To further study the transcription profile of mutant M1 and its control (BY-P41K-RPB7), we have employed whole genome microarray expression profiling as a discovery platform to identify differentially expressed genes in YPAD medium under OD600 reached 1 and after 12 VHG fermentation.
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| Overall design |
Total cellular RNAs were extracted from both the mutant and the control using RNeasy® Mini Kit and RNase-Free DNase Set (Qiagen,Hilden, Germany) under the following two conditions: i) when cells reached early exponential phase (OD600 ~1.0) in YPAD; ii) after 12h VHG fermentation. RNA quality and integrity was verified by gel electrophoresis, as well as by measuring 260/230 ratios with a NanoDrop 1000 spectrophotometer (Thermo Scientific, MA, USA). Two biological replicates of each sample were sent to Genomax Technologies (Singapore) for DNA microarray assay using Yeast (V2) Gene Expression Microarray, 8×15K Microarrays (Agilent technologies, USA). The obtained data was analyzed by Agilent Genespring GX software and the p-values were calculated by unpaired Student t-test.
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| Contributor(s) |
Zilong Q |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 11, 2016 |
| Last update date |
May 18, 2017 |
| Contact name |
Zilong Qiu |
| E-mail(s) |
tom0811090@aliyun.com
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| Organization name |
Nanyang Technological University
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| Department |
School of Chemical & Biomedical Engineering
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| Street address |
62 Nanyang Drive
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| City |
Singapore |
| ZIP/Postal code |
637459 |
| Country |
Singapore |
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| Platforms (1) |
| GPL16244 |
Agilent-016322 Yeast (V2) Gene Expression 8x15K Microarray (Probe Name version) |
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| Samples (8)
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| GSM2060472 |
BY-P41K- Rpb7 after 12h VHG fermentation sample 1 |
| GSM2060473 |
M1 after 12h VHG fermentation sample 1 |
| GSM2060474 |
BY-P41K- Rpb7 after 12h VHG fermentation sample 2 |
| GSM2060475 |
M1 after 12h VHG fermentation sample 2 |
| GSM2060476 |
M1 growth in YPAD until OD reached 1 sample 1 |
| GSM2060477 |
BY-P41K- Rpb7 growth in YPAD until OD reached 1 sample 1 |
| GSM2060478 |
M1 growth in YPAD until OD reached 1 sample 2 |
| GSM2060479 |
BY-P41K- Rpb7 growth in YPAD until OD reached 1 sample 2 |
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| Relations |
| BioProject |
PRJNA311732 |
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