|
| Status |
Public on Feb 12, 2016 |
| Title |
BY-P41K- Rpb7 after 12h VHG fermentation sample 2 |
| Sample type |
RNA |
| |
|
| Source name |
12h VHG fermentation
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
genotype/variation: BY-P41K- Rpb7
treatment: 12h VHG fermentation
|
| Treatment protocol |
There are two conditions: i) when cells reached early exponential phase (OD600 ~1.0) in YPAD; ii) after 12h VHG fermentation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total cellular RNAs were extracted from both the mutant and the control using RNeasy® Mini Kit and RNase-Free DNase Set (Qiagen,Hilden, Germany)
|
| Label |
cyanine 3-CTP
|
| Label protocol |
Low Input Quick Amp Labeling Kit (Agilent: 5190-2331), Lot: 0006208922
|
| |
|
| Hybridization protocol |
Gene Expression Hybridization Kit (5188-5242), Lot: 0006279504
|
| Scan protocol |
After hybridization, the microarray slide was washed in gene expression wash buffer 1 for one minute at room temperature and another minute in gene expression wash buffer 2 at 37oC before scanning on Agilent High Resolution Microarray Scanner (C-model).
|
| Description |
Gene expression after 12h VHG fermentation
|
| Data processing |
Raw signal data were extracted from the TIFF image with Agilent Feature Extraction Software (V10.7.1.1).
|
| |
|
| Submission date |
Feb 11, 2016 |
| Last update date |
Feb 12, 2016 |
| Contact name |
Zilong Qiu |
| E-mail(s) |
tom0811090@aliyun.com
|
| Organization name |
Nanyang Technological University
|
| Department |
School of Chemical & Biomedical Engineering
|
| Street address |
62 Nanyang Drive
|
| City |
Singapore |
| ZIP/Postal code |
637459 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL16244 |
| Series (1) |
| GSE77853 |
Gene expression change of M1 and BY-P41K-RPB7 in YPAD (OD600 reached 1) and after 12h Very High Gravity (VHG) fermentation |
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