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Status |
Public on Jul 11, 2007 |
Title |
Identification of genes enriched in putative stem/progenitor cells from mouse embryonic pancreas |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
Identification of genes enriched in putative stem/progenitor cells (CD133highPDGFRb- cell population) from the mouse embryonic pancreas that are purified by fluorescence activated cell sorting (FACS).
Success in islet transplantation-based therapies for type 1 diabetes mellitus and an extreme shortage of pancreatic islets has motivated efforts to develop renewable sources of islet-replacement tissue. Only a few attempts have been made at prospective isolation of pancreatic stem/progenitor cells, due to the lack of specific markers and the development of cell culture method. This study demonstrates the isolation of pancreatic stem/progenitor cells from the embryonic pancreas by cell sorting. RT-PCR and microarray analysis demonstrated that pancreatic stem/progenitor cells are enriched in CD133highPDGFRb- cell population. During in vivo differentiation, these cell populations have the ability for self-renewal and multipotency, including the formation of insulin-producing cells. Since the strategy is based on the cell sorting using cell surface markers common to human and rodents, it may promote strategies to derive transplantable islet-replacement tissues from human pancreatic stem/progenitor cells. Keywords: Cell type comparison
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Overall design |
A couple of total RNAs derived from the different status of PDGFRb- cells was subjected to a dual-color microarray analysis, in which the RNAs from CD133highPDGFRb- cell population was labeled with Cy3 and that of CD133negPDGFRb- cell population with Cy5 respectively.
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Contributor(s) |
Hori Y, Fukumoto M, Kuroda Y |
Citation(s) |
18703663 |
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Submission date |
May 15, 2007 |
Last update date |
May 10, 2018 |
Contact name |
Yuichi Hori |
E-mail(s) |
horiy@med.kobe-u.ac.jp
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Phone |
+81-78-382-5371
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Fax |
+81-78-382-5379
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Organization name |
Kobe University School of Medicine
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Department |
Surgery
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Street address |
7-5-1 Kusunoki-cho, Chuo-ku
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City |
Kobe |
State/province |
Hyogo |
ZIP/Postal code |
650-0017 |
Country |
Japan |
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Platforms (1) |
GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
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Samples (1) |
GSM189373 |
Murine embyonic pancreas stem cells |
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Relations |
BioProject |
PRJNA99833 |