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Series GSE7800 Query DataSets for GSE7800
Status Public on Jul 11, 2007
Title Identification of genes enriched in putative stem/progenitor cells from mouse embryonic pancreas
Organism Mus musculus
Experiment type Expression profiling by array
Summary Identification of genes enriched in putative stem/progenitor cells (CD133highPDGFRb- cell population) from the mouse embryonic pancreas that are purified by fluorescence activated cell sorting (FACS).

Success in islet transplantation-based therapies for type 1 diabetes mellitus and an extreme shortage of pancreatic islets has motivated efforts to develop renewable sources of islet-replacement tissue. Only a few attempts have been made at prospective isolation of pancreatic stem/progenitor cells, due to the lack of specific markers and the development of cell culture method. This study demonstrates the isolation of pancreatic stem/progenitor cells from the embryonic pancreas by cell sorting. RT-PCR and microarray analysis demonstrated that pancreatic stem/progenitor cells are enriched in CD133highPDGFRb- cell population. During in vivo differentiation, these cell populations have the ability for self-renewal and multipotency, including the formation of insulin-producing cells. Since the strategy is based on the cell sorting using cell surface markers common to human and rodents, it may promote strategies to derive transplantable islet-replacement tissues from human pancreatic stem/progenitor cells.
Keywords: Cell type comparison
 
Overall design A couple of total RNAs derived from the different status of PDGFRb- cells was subjected to a dual-color microarray analysis, in which the RNAs from CD133highPDGFRb- cell population was labeled with Cy3 and that of CD133negPDGFRb- cell population with Cy5 respectively.
 
Contributor(s) Hori Y, Fukumoto M, Kuroda Y
Citation(s) 18703663
Submission date May 15, 2007
Last update date May 10, 2018
Contact name Yuichi Hori
E-mail(s) horiy@med.kobe-u.ac.jp
Phone +81-78-382-5371
Fax +81-78-382-5379
Organization name Kobe University School of Medicine
Department Surgery
Street address 7-5-1 Kusunoki-cho, Chuo-ku
City Kobe
State/province Hyogo
ZIP/Postal code 650-0017
Country Japan
 
Platforms (1)
GPL4134 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version)
Samples (1)
GSM189373 Murine embyonic pancreas stem cells
Relations
BioProject PRJNA99833

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE7800_RAW.tar 19.4 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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