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| Status |
Public on Mar 28, 2008 |
| Title |
Assessment of CcpA-mediated catabolite control of metabolism and enterotoxin production in Bacillus cereus ATCC 14579 |
| Organism |
Bacillus cereus ATCC 14579 |
| Experiment type |
Expression profiling by array
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| Summary |
In Bacillus cereus the catabolite control protein CcpA was shown to be involved in optimizing the efficiency of glucose catabolism by activating genes encoding glycolytic enzymes including a non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase that mediates conversion of D-glyceraldehyde 3-phosphate to 3-phospho-D-glycerate in one single step, and by repressing genes encoding the citric acid cycle and gluconeogenic enzymes. Two B. cereus-specific CcpA-regulated operons were identified, encoding enzymes involved in the catabolism of fuculose/arabinose and aspartate. In addition, a genome search using the CRE-site consensus predicted the B. cereus CcpA regulon to include 10 PTS-system gene clusters as well as genes coding for overflow metabolic enzymes leading to acetoin and acetate. Notably, catabolite repression of the genes encoding non-hemolytic enterotoxin (Nhe) and hemolytic (Hbl) enterotoxin appeared CcpA-dependent, and for the corresponding enterotoxin operons, putative CRE-sites were identified. This points to metabolic control of enterotoxin gene expression and suggests that CcpA-mediated glucose sensing provides an additional mode of control to PlcR activated expression of nhe and hbl genes in B. cereus.
Keywords: Time course analysis by comparing transriptomes of the wildtype and the ccpA deleton strain.
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| Overall design |
The wildtype (B. cereus ATCC 14579) and ccpA deletion strain were sampled during aerobic growth in Brain heart infusion broth. Samples of wildtype and ccpA deletion strain were compared at 4 time points, i.e. early exponential (OD600 0.2), mid-exponential (OD600 0.8), transition phase (OD600 4), and stationary phase (OD600 8). For each time point biological duplo's were obtained, which were subsequently differently labelled to perform a dye swap.
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| Contributor(s) |
van der Voort M, Kuipers OP, Buist G, de Vos WM, Abee T |
| Citation(s) |
18416820 |
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| Submission date |
May 18, 2007 |
| Last update date |
Mar 17, 2012 |
| Contact name |
Menno van der Voort |
| Organization name |
Wageningen UR
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| Department |
Agrotechnology and Food Sciences Group
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| Lab |
Food Microbiology
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| Street address |
Bomenweg 2
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| City |
Wageningen |
| ZIP/Postal code |
6703 HD |
| Country |
Netherlands |
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| Platforms (1) |
| GPL5161 |
Bacillus cereus ATCC 14579 amplicon-based 12k microarray |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA99971 |
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