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| Status |
Public on Jun 20, 2007 |
| Title |
profiling of the root architecture response to phyllobacterium-Arabidopsis thaliana transcriptome analysis in ... |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
ra04-07_pgpr - profiling of the root architecture response to phyllobacterium - Experiment 1 : Which genes are up- or down-regulated in Arabidopsis thaliana cultivated in vitro with increased lateral root development in response to Phyllobacterium STM196 inoculation. Experiment 2 : Which genes are up- or down-regulated during the ISR triggered by a rhizobacteria, in comparison with those affected by a pathogenic interaction. Experiment 3 : which genes are specifically induced or repressed in Arabidopsis thaliana by inoculation of the soil with a PGPR vs a bacteria that has the ability to trigger nodule formation in a Legume. - Seeds of wild-type Arabidopsis thaliana (ecotype Columbia) were surface-sterilized and sawn on agar mineral medium (see below). 4 days after storage in the dark at 4degreeC, seedling were cultivated 6 days in a growth chamber (16 h daily, 20-22degreeC) and then transferred on a fresh agar mineral medium inoculated or not with Phyllobacterium STM196 (2.108 cfu/ml). 6 days later, root and leaves were collected, froze on liquid nitrogen and stored at -80degreeC.
Keywords: treated vs untreated comparison
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| |
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| Overall design |
2 dye-swap - CATMA arrays
|
| Web link |
http://urgv.evry.inra.fr/cgi-bin/projects/CATdb/consult_expce.pl?experiment_id=62
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| |
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| Contributor(s) |
Taconnat L, Renou JP, Touraine B, Martin-Magniette ML |
| Citation(s) |
18184068 |
| |
| Submission date |
Jun 01, 2007 |
| Last update date |
Mar 17, 2012 |
| Contact name |
Véronique BRUNAUD |
| E-mail(s) |
veronique.brunaud@inrae.fr
|
| Organization name |
INRA - CNRS - UPSUD
|
| Lab |
IPS2
|
| Street address |
rue Noetzlin
|
| City |
Gif-sur-Yvette |
| ZIP/Postal code |
91190 |
| Country |
France |
| |
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| Platforms (1) |
| GPL5337 |
URGV Arabidopsis thaliana 25K CATMA_v2.1 |
|
| Samples (4)
|
| GSM197556 |
12620323 - Root Phyllo vs Control Root Phyllo |
| GSM197557 |
12961235 - Control Root Phyllo vs Root Phyllo |
| GSM197558 |
12625723 - Control Leaf Phyllo vs Leaf Phyllo |
| GSM197559 |
12620586 - Leaf Phyllo vs Control Leaf Phyllo |
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| Relations |
| BioProject |
PRJNA100769 |
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