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Series GSE80831 Query DataSets for GSE80831
Status Public on Aug 10, 2016
Title A Basic Domain in the Histone H2B N-terminal Tail is Important for Nucleosome Assembly by FACT
Organism Saccharomyces cerevisiae
Experiment type Genome binding/occupancy profiling by genome tiling array
Summary Nucleosome assembly in vivo requires assembly factors, such as histone chaperones, to bind to histones and mediate their deposition onto DNA. In yeast, the essential histone chaperone FACT (FAcilitates Chromatin Transcription) functions in nucleosome assembly and H2A-H2B deposition during transcription elongation and DNA replication. Recent studies have identified candidate histone residues that mediate FACT binding to histones, but it is not known which histone residues are important for FACT to deposit histones onto DNA during nucleosome assembly. In this study, we report that the histone H2B repression (HBR) domain within the H2B N-terminal tail is important for histone deposition by FACT. Deletion of the HBR domain causes significant defects in histone occupancy in the yeast genome, particularly at HBR-repressed genes, and a pronounced increase in H2A-H2B dimers that remain bound to FACT in vivo. Moreover, the HBR domain is required for purified FACT to efficiently assemble recombinant nucleosomes in vitro. We propose that the interaction between the highly basic HBR domain and DNA plays an important role in stabilizing the nascent nucleosome during the process of histone H2A-H2B deposition by FACT.
Overall design 6 samples, 2 inputs and 4 ChIP samples for histone H2B (2 for wild-type and 2 for an H2B ∆30-37 mutant)
Contributor(s) Wyrick JJ, Kyriss M
Citation(s) 27369377
Submission date Apr 29, 2016
Last update date Aug 10, 2016
Contact name John J. Wyrick
Phone 509-335-8785
Organization name Washington State University
Department School of Molecular Biosciences
Street address Biotechnology Life Sciences 241
City Pullman
State/province WA
ZIP/Postal code 99164
Country USA
Platforms (1)
GPL7250 [Sc03b_MR] Affymetrix GeneChip S. cerevisiae Tiling 1.0R Array
Samples (6)
GSM2138564 WT H2B IP - replicate 1
GSM2138565 WT H2B IP - replicate 2
GSM2138566 WT Input
BioProject PRJNA320099

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE80831_RAW.tar 210.6 Mb (http)(custom) TAR (of CEL, WIG)
Processed data provided as supplementary file

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