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| Status |
Public on Aug 10, 2016 |
| Title |
A Basic Domain in the Histone H2B N-terminal Tail is Important for Nucleosome Assembly by FACT |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
Nucleosome assembly in vivo requires assembly factors, such as histone chaperones, to bind to histones and mediate their deposition onto DNA. In yeast, the essential histone chaperone FACT (FAcilitates Chromatin Transcription) functions in nucleosome assembly and H2A-H2B deposition during transcription elongation and DNA replication. Recent studies have identified candidate histone residues that mediate FACT binding to histones, but it is not known which histone residues are important for FACT to deposit histones onto DNA during nucleosome assembly. In this study, we report that the histone H2B repression (HBR) domain within the H2B N-terminal tail is important for histone deposition by FACT. Deletion of the HBR domain causes significant defects in histone occupancy in the yeast genome, particularly at HBR-repressed genes, and a pronounced increase in H2A-H2B dimers that remain bound to FACT in vivo. Moreover, the HBR domain is required for purified FACT to efficiently assemble recombinant nucleosomes in vitro. We propose that the interaction between the highly basic HBR domain and DNA plays an important role in stabilizing the nascent nucleosome during the process of histone H2A-H2B deposition by FACT.
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| Overall design |
6 samples, 2 inputs and 4 ChIP samples for histone H2B (2 for wild-type and 2 for an H2B ∆30-37 mutant)
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| Contributor(s) |
Wyrick JJ, Kyriss M |
| Citation(s) |
27369377 |
| |
| Submission date |
Apr 29, 2016 |
| Last update date |
Aug 10, 2016 |
| Contact name |
John J. Wyrick |
| E-mail(s) |
jwyrick@vetmed.wsu.edu
|
| Phone |
509-335-8785
|
| Organization name |
Washington State University
|
| Department |
School of Molecular Biosciences
|
| Street address |
Biotechnology Life Sciences 241
|
| City |
Pullman |
| State/province |
WA |
| ZIP/Postal code |
99164 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL7250 |
[Sc03b_MR] Affymetrix GeneChip S. cerevisiae Tiling 1.0R Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA320099 |
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