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Series GSE80957 Query DataSets for GSE80957
Status Public on Dec 27, 2017
Title Comparative analysis of the transcriptome responses of zebrafish embryos after exposure to low concentrations of essential and non-essential metals.
Organism Danio rerio
Experiment type Expression profiling by array
Summary Metal toxicity is a global environmental challenge. Fish are particularly prone to metal exposure, which can be lethal or can cause sublethal physiological impairments. The objective of this study was to improve our understanding on how adverse effects of essential and non-essential metals in early life stage zebrafish may be explained by changes in the transcriptome after exposure to non-toxic levels. We therefore studied the effects of three different metals at low concentrations in zebrafish embryos by transcriptomics analysis. The study design compared exposure effects caused by different metals at different developmental stages (pre-hatch and post-hatch). Wild-type embryos were exposed to solutions of low concentrations of copper (CuSO4), cadmium (CdCl2) and cobalt (CoSO4) until 96 h post-fertilization (hpf) and microarray experiments were carried out to determine transcriptome profiles at 48 and 96 hpf. We found that the toxic metal cadmium affected the expression of more genes at 96 hpf than 48 hpf. The opposite effect was observed for the essential metals cobalt and copper, which also showed enrichment of different GO terms. Genes involved in neuromast and motor neuron development, were significantly enriched, agreeing with our previous results showing motor neuron and neuromast damage in the embryos. Our data provide evidence that the response of the transcriptome of fish embryos to metal exposure differs for essential and non-essential metals.
 
Overall design Zebrafish embryos exposed to 3.3 mg/L cadmium treatment (C3), 3.6 mg/L cobalt treatment (C3) and 6.1 µg/L copper treatment (C3) with additional ISO water controls (C0); Two developmental stages 48 hpf and 96 hpf; 48 hpf three replicates and 96 hpf four replicates
 
Contributor(s) Sonnack L, Klawonn T, Kiehuber R, Hollert H, Schäfers C, Fenske M
Citation(s) 29287281
Submission date Apr 29, 2016
Last update date Aug 09, 2018
Contact name Laura Sonnack
E-mail(s) laura.sonnack@rwth-aachen.de
Organization name Fraunhofer IME
Street address Forckenbeckstr. 6
City Aachen
ZIP/Postal code 52074
Country Germany
 
Platforms (1)
GPL14688 Agilent-026437 D. rerio (Zebrafish) Oligo Microarray V3 (Feature Number version)
Samples (42)
GSM2138972 Cadmium 48h control replicate 1
GSM2138973 Cadmium 48h control replicate 2
GSM2138974 Cadmium 48h control replicate 3
Relations
BioProject PRJNA320113

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE80957_RAW.tar 381.6 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Processed data provided as supplementary file

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