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| Status |
Public on Dec 01, 2016 |
| Title |
Alfa2-COP is involved in early secretory traffic in Arabidopsis and it is required for plant growth |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
The loss-of-function mutants of alfa1-COP (alfa1-COP-1) and alfa2-COP (alfa2-COP1), the two alfa-COP isoforms of Arabidopsis. Unlike in alfa1-COP mutant, the alfa2-COP mutant displays defects in plant growth, including small rosettes, stems and roots and mislocalization of p24a5, a protein containing a C-terminal dylisine motif involved in COPI binding. In addition, it exhibited abnormal morphology of the Golgi apparatus. Global expression analyses of the alfa2-COP mutant versus wild-type Arabidopsis plants reveal altered expression of plant cell wall-associated genes. A high expression of the COPII sec31A isoform was observed in the alfa2-COP mutant that also occurs in a mutant of an upstream gene of COPI assembly, the ARF-GEF GNL1
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| Overall design |
Four biological replicates were generated. The goal of this experiment is to compared the α2-COP-3 mutant against a Col-0 wild type line. In the comparison 2 of the replicates were reversed-labeled.
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| Contributor(s) |
Gimeno-Ferrer F, Pastor-Cantizano N, Bernat-Silvestre C, Selvi-Martinez P, Vera-Sirera F, Gao C, Perez-Amador MA, Jiang L, Aniento F, Marcote MJ |
| Citation(s) |
28013293, 28025315 |
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| Submission date |
May 03, 2016 |
| Last update date |
Sep 25, 2019 |
| Contact name |
Francisco Vera-Sirera |
| E-mail(s) |
fravesi@ibmcp.upv.es
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| Organization name |
IBMCP (UPV-CSIC)
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| Lab |
Lab 2.08
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| Street address |
Ingeniero Fausto Elio, s/n
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| City |
Valencia |
| ZIP/Postal code |
46022 |
| Country |
Spain |
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| Platforms (1) |
| GPL9020 |
Agilent-021169 Arabidopsis 4 Oligo Microarray (V4) (Feature Number version) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA320381 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE81049_RAW.tar |
58.2 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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