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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Aug 01, 2016 |
| Title |
Expression data from Tbet+ and Tbet- Memory B cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The role of antibody and B cells in preventing infection is established. In contrast, the role of B cell responses in containing chronic infections remains poorly understood. IgG2a (IgG1 in humans) can prevent acute infections and T-bet promotes IgG2a isotype switching. However, whether IgG2a and B cell-expressed T-bet influence the host-pathogen balance during persisting infections is unclear. Here we demonstrate that B cell specific loss of T-bet prevents control of persisting viral infection. T-bet in B cells not only controlled IgG2a production, but also mucosal localization, proliferation, glycosylation, and a broad transcriptional program. T-bet controlled a broad antiviral program in addition to IgG2a since T-bet in B cells was important even in the presence of virus-specific IgG2a. Our data supports a model in which T-bet is a universal controller of antiviral immunity across multiple immune lineages.
Naïve, Tbet+, and Tbet- Memory B cells were assayed for gene expression
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| Overall design |
Tbet GFP reporter mice were infected with LCMV clone 13, and target B cell populations were sorted from splenocytes at day 10 post-infection
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| Contributor(s) |
Barnett BE, Stelekati E, Wherry EJ |
| Citation(s) |
27430722 |
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| Submission date |
May 06, 2016 |
| Last update date |
Feb 21, 2018 |
| Contact name |
Burton Earle Barnett |
| E-mail(s) |
burtonbarnett@gmail.com
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| Organization name |
Poseida Therapeutics
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| Street address |
3210 Merryfield Row
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| City |
San Diego |
| State/province |
California |
| ZIP/Postal code |
92121 |
| Country |
USA |
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| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA320853 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE81189_RAW.tar |
79.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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