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| Status |
Public on Oct 31, 2016 |
| Title |
DUAL RNASeq ANALYSIS REVEALS AN INCREASE OF FUNGAL PATHOGENICITY FUNCTIONS AND A ROLE OF PLANT GLUTAMINE SYNTHETASE IN NITROGEN-INDUCED SUSCEPTIBILITY TO THE RICE BLAST FUNGUS |
| Organisms |
Oryza sativa; Pyricularia oryzae |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In order to understand the mechanisms of Nitrogen induced susceptibility (NIS) we’ve conducted a dual RNAseq experiment on rice infected tissues by Magnaporthe oryzae. At 0 day post inoculation and 2 days post inoculation tissues have been collected on mock inoculated and M. oryzae inoculated plants. Rice were conducted under two type of nitrogen fertilization: 0N all fertilization but nitrogen, 1N all fertilization and NH4NO3. The fertilization was applied one day before inoculation. RNAseq was conducted both on rice and fungal RNA.
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| Overall design |
shoot tissus mRNA 28-day old rice plant were generated by deep sequencing. Three samples per conditions, Four conditions: 0N Mock, 0N Inoc, 1N Mock, 1N Inoc. 0N = plant fertilized one day before inoculation without nitrogen. 1N = plant fertilized one day before inoculation with NH4NO3. Mock = Plant inoculated with control solution (gelatin 0,5%). Inoc = Plant inoculated with Magnaporthe oryzae isolate Guy11 with gelatin 0,5%.
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| Contributor(s) |
BALLINI E, HUANG H, HE X, MOREL J |
| Citation missing |
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| |
| Submission date |
Jun 10, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Elsa Ballini |
| E-mail(s) |
elsa.ballini@supagro.fr
|
| Phone |
0033499624817
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| Organization name |
SupAgro Montpellier
|
| Department |
Biology and ecology
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| Lab |
UMR BGPI/ICAP
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| Street address |
UMR BGPI – TA A-54/K – Equipe ICAP campus International de Baillarguet
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| City |
Montpellier cedex 5 |
| ZIP/Postal code |
34398 |
| Country |
France |
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| Platforms (2) |
| GPL13160 |
Illumina HiSeq 2000 (Oryza sativa) |
| GPL16530 |
Illumina HiSeq 2000 (Magnaporthe oryzae) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA325291 |
| SRA |
SRP076382 |
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