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Status |
Public on Oct 31, 2016 |
Title |
Ethylene induced phosphorylation of CBP20 is involved in root growth [RNA-seq] |
Organism |
Arabidopsis thaliana |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We report that CBP20 phosphorylation can regulate root growth in ethylene. We examined the gene expression in roots and shoots of wild type (Col) and cbp20 mutant (in Col background). Ethylene is one of the most essential hormones for plant developmental processes and stress responses. EIN2 is a key factor in ethylene signaling pathway and its function is regulated by phosphorylation. However, the phosphorylation regulation in the ethylene signaling pathway is largely unknown. Here we report the phosphorylation of CBP20 is regulated by ethylene, and the phosphorylation is involved in root elongation. The constitutive phosphorylation format of CBP20 rescues the cbp20 root ethylene hyposensitive phenotype, while the constitutive de-phosphorylation format of CBP20 is unable to rescue the root phenotype of cbp20 in response to ethylene. Genome wide study on ethylene regulated gene expression and microRNA expression in the roots and shoots of both Col and cbp20, together with the result of genetics validation suggest that ethylene induced phosphorylation of CBP20 is involved in root growth and one pathway is through the regulation of microRNAs and their target genes in roots.
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Overall design |
Root and shoot mRNA profiles of 3-day old wild type (Col) and cbp20 mutant (in Col background) were generated by sequencing, in 2 replications, using Illumina HiSeq 4000
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Contributor(s) |
Qiao H, Zhang F, Wang L |
Citation(s) |
27870849 |
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Submission date |
Jun 21, 2016 |
Last update date |
May 15, 2019 |
Contact name |
FAN ZHANG |
E-mail(s) |
biozhangfancn@outlook.com
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Organization name |
The University of Texas at Austin
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Street address |
2506 speedway, NMS5.324
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City |
Austin |
ZIP/Postal code |
78712 |
Country |
USA |
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Platforms (1) |
GPL21785 |
Illumina HiSeq 4000 (Arabidopsis thaliana) |
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Samples (16)
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GSM2209780 |
Col shoot 4 hour ethylene RNAseq, rep2 |
GSM2209781 |
cbp20 shoot 4 hour air RNAseq, rep1 |
GSM2209782 |
cbp20 shoot 4 hour air RNAseq, rep2 |
GSM2209783 |
cbp20 shoot 4 hour ethylene RNAseq, rep1 |
GSM2209784 |
cbp20 shoot 4 hour ethylene RNAseq, rep2 |
GSM2209785 |
Col root 4 hour air RNAseq, rep1 |
GSM2209786 |
Col root 4 hour air RNAseq, rep2 |
GSM2209787 |
Col root 4 hour ethylene RNAseq, rep1 |
GSM2209788 |
Col root 4 hour ethylene RNAseq, rep2 |
GSM2209789 |
cbp20 root 4 hour air RNAseq, rep1 |
GSM2209790 |
cbp20 root 4 hour air RNAseq, rep2 |
GSM2209791 |
cbp20 root 4 hour ethylene RNAseq, rep1 |
GSM2209792 |
cbp20 root 4 hour ethylene RNAseq, rep2 |
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This SubSeries is part of SuperSeries: |
GSE83575 |
Phosphorylation of CBP20 links microRNA to root growth in the ethylene response |
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Relations |
BioProject |
PRJNA326409 |
SRA |
SRP076862 |
Supplementary file |
Size |
Download |
File type/resource |
GSE83573_COL_root_airvsC2H4.xls.gz |
798.2 Kb |
(ftp)(http) |
XLS |
GSE83573_COL_shoot_airvsC2H4.xls.gz |
796.6 Kb |
(ftp)(http) |
XLS |
GSE83573_cbp20_root_airvsC2H4.xls.gz |
811.7 Kb |
(ftp)(http) |
XLS |
GSE83573_cbp20_shoot_airvsC2H4.xls.gz |
800.2 Kb |
(ftp)(http) |
XLS |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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