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Series GSE83573 Query DataSets for GSE83573
Status Public on Oct 31, 2016
Title Ethylene induced phosphorylation of CBP20 is involved in root growth [RNA-seq]
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary We report that CBP20 phosphorylation can regulate root growth in ethylene. We examined the gene expression in roots and shoots of wild type (Col) and cbp20 mutant (in Col background).
Ethylene is one of the most essential hormones for plant developmental processes and stress responses. EIN2 is a key factor in ethylene signaling pathway and its function is regulated by phosphorylation. However, the phosphorylation regulation in the ethylene signaling pathway is largely unknown. Here we report the phosphorylation of CBP20 is regulated by ethylene, and the phosphorylation is involved in root elongation. The constitutive phosphorylation format of CBP20 rescues the cbp20 root ethylene hyposensitive phenotype, while the constitutive de-phosphorylation format of CBP20 is unable to rescue the root phenotype of cbp20 in response to ethylene. Genome wide study on ethylene regulated gene expression and microRNA expression in the roots and shoots of both Col and cbp20, together with the result of genetics validation suggest that ethylene induced phosphorylation of CBP20 is involved in root growth and one pathway is through the regulation of microRNAs and their target genes in roots.
 
Overall design Root and shoot mRNA profiles of 3-day old wild type (Col) and cbp20 mutant (in Col background) were generated by sequencing, in 2 replications, using Illumina HiSeq 4000
 
Contributor(s) Qiao H, Zhang F, Wang L
Citation(s) 27870849
Submission date Jun 21, 2016
Last update date May 15, 2019
Contact name FAN ZHANG
E-mail(s) biozhangfancn@outlook.com
Organization name The University of Texas at Austin
Street address 2506 speedway, NMS5.324
City Austin
ZIP/Postal code 78712
Country USA
 
Platforms (1)
GPL21785 Illumina HiSeq 4000 (Arabidopsis thaliana)
Samples (16)
GSM2209777 Col shoot 4 hour air RNAseq, rep1
GSM2209778 Col shoot 4 hour air RNAseq, rep2
GSM2209779 Col shoot 4 hour ethylene RNAseq, rep1
This SubSeries is part of SuperSeries:
GSE83575 Phosphorylation of CBP20 links microRNA to root growth in the ethylene response
Relations
BioProject PRJNA326409
SRA SRP076862

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SOFT formatted family file(s) SOFTHelp
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Supplementary file Size Download File type/resource
GSE83573_COL_root_airvsC2H4.xls.gz 798.2 Kb (ftp)(http) XLS
GSE83573_COL_shoot_airvsC2H4.xls.gz 796.6 Kb (ftp)(http) XLS
GSE83573_cbp20_root_airvsC2H4.xls.gz 811.7 Kb (ftp)(http) XLS
GSE83573_cbp20_shoot_airvsC2H4.xls.gz 800.2 Kb (ftp)(http) XLS
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