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| Status |
Public on Jan 01, 2017 |
| Title |
Genome-wide Transcriptome Analysis of CD36 Overexpression in HepG2.2.15 Cells to Explore Its Regulation Role of Metabolism and HBV Life Cycle |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To understand the molecular basis underlying the response to increasing CD36 overexpression in HepG2.2.15 cell, we performed genome-wide sequencing of the mRNAs from HepG2.2.15-CD36OE cell and HepG2.2.15-vector cell using the RNA-seq technology to detect its differential transcriptomic profile.
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| Overall design |
To better understand the molecular changes in the response of the CD36 overexpression in HepG2.2.15 cell, three cDNA libraries from HepG2.2.15-vector cell (Vector-1, Vector-2, Vector-3) and HepG2.2.15-CD36 overexpression cell (CD36OE-1, CD36OE-2, CD36OE-3) were constructed, respectively. After ensuring quality control, Ion ProtonTM Sequencer was used for sequencing the cDNA libraries.
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| Contributor(s) |
Huang J, Chen Y |
| Citation(s) |
27749922 |
| |
| Submission date |
Jun 21, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
健 黄 |
| E-mail(s) |
zmchj2001@163.com
|
| Phone |
15086067610
|
| Organization name |
遵义医学院附属医院
|
| Street address |
汇川区大连路149号遵义医学院附属医院
|
| City |
遵义市 |
| State/province |
贵州省 |
| ZIP/Postal code |
563003 |
| Country |
China |
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| Platforms (1) |
| GPL17303 |
Ion Torrent Proton (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA326403 |
| SRA |
SRP076864 |
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