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| Status |
Public on Jan 17, 2017 |
| Title |
Deep sequencing of wheat sRNA transcriptome reveals distinct temporal expression pattern of miRNAs in response to heat, light and UV |
| Organism |
Triticum aestivum |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Understanding of plant adaptation to abiotic stresses has implications in plant breeding, especially in the context of climate change. MicroRNAs (miRNAs) and short interfering RNAs play a crucial role in gene regulation. Here, wheat plants were exposed to one of the following stresses: continuous light, heat or ultraviolet radiations over five consecutive days and, leaf tissues from three biological replicates were harvested at 0, 1, 2, 3, 7 and 10 days after treatment (DAT). A total of 72 small RNA libraries were sequenced on the Illumina platform generating ~524 million reads corresponding to ~129 million distinct tags from which 232 conserved miRNAs were identified. The expression levels of 1, 2 and 79 miRNAs were affected by ultraviolet radiations, continuous light and heat, respectively. Approximately 55% of the differentially expressed miRNAs were downregulated at 0 and 1 DAT including miR398, miR528 and miR156 that control mRNAs involved in activation of signal transduction pathways and flowering. Other putative targets included histone variants and methyltransferases. These results suggest a temporal miRNA-guided post-transcriptional regulation that enables wheat to respond to abiotic stresses, particularly heat. Designing novel wheat breeding strategies such as regulatory gene-based marker assisted selection depends on accurate identification of stress induced miRNAs.
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| Overall design |
Wheat plants were exposed to one of the following stresses: continuous light, heat or ultraviolet radiations over five consecutive days and, leaf tissues from three biological replicates were harvested at 0, 1, 2, 3, 7 and 10 days after treatment (DAT). A total of 72 small RNA libraries were sequenced on the Illumina platform
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| Contributor(s) |
Ragupathy R, Ravichandran S, Mahdi SR, Huang D, Reimer E, Domaratzki M, Cloutier S |
| Citation(s) |
28004741 |
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| Submission date |
Jun 27, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Sylvie Cloutier |
| E-mail(s) |
sylviej.cloutier@canada.ca
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| Organization name |
Agriculture and Agri-Food Canada
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| Street address |
960 Carling Avenue
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| City |
Ottawa |
| State/province |
Ontario |
| ZIP/Postal code |
K1A 0C6 |
| Country |
Canada |
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| Platforms (1) |
| GPL17701 |
Illumina HiSeq 2000 (Triticum aestivum) |
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| Samples (72)
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| Relations |
| BioProject |
PRJNA326902 |
| SRA |
SRP077258 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE83738_Conserved_miRNA_families_and_their_coresponding_read_counts.xls.gz |
198.5 Kb |
(ftp)(http) |
XLS |
SRA Run Selector |
| Processed data are available on Series record |
| Raw data are available in SRA |
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