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| Status |
Public on Jul 11, 2017 |
| Title |
Binary polycyclic aromatic hydrocarbon exposure alters the transcriptome inducing inflammation and inhibiting cell communication through p38 MAPK in lung cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Low molecular weight polycyclic aromatic hydrocarbons (LMW PAHs) are generated by the incomplete combustion of organic material and are prevalent and ubiquitous in the environment, presenting a human health concern. While much is known about the high molecular weight genotoxic species of PAHs, there are insufficient studies for LMW PAHs and their impact on pulmonary health. Secondhand smoke, or environmental tobacco smoke (ETS), is a mixture of many PAHs, among other constituents. To study the effect of more than one PAH, we examined single PAH and a binary mixture on the dysregulation of gap junctional intercellular communication (GJIC), activation of mitogen activated protein kinases (MAPK), and induction of inflammation with a focus on the p38 MAPK pathway through transcriptomic analysis. A mouse non-tumorigenic alveolar type II cell line, C10, along with two normal, non-tumorigenic human bronchial epithelial cell lines, Beas2b and HBE1 cells, for human comparisons. Our findings in all cell lines indicate that 1-methylanthracene (1-MeA) and fluoranthene (Flthn), can dysregulated GJIC in a dose dependent manner and combining these two compounds lead to a similar effect on GJIC. MAPKs, particularly P38, are rapidly activated and the inhibition of this MAPK leads to a reversal of PAH dysregulation of GJIC. A toxicogenomic approach conducted using the C10 cells revealed inflammatory, metabolic, steroid synthesis, and oxidative pathways altered synergistically by a mixture of PAHs (1-MeA and Flthn), followed by qRT-PCR, ELISAs, and immunoblots validation. Our findings indicated LMW PAHs potential to induce pulmonary inflammation and emphasize the need for mixture exposure research to accurately estimate human exposure risk.
Grant ID: CIA130022
Grant Title: Components of secondhand smoke in COPD.
Funding Source: Flight Attendant Medical Research Institute
Name: Alison,K,Bauer
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| Overall design |
A mouse alveolar type II cell line was used for this study called C10 cells. Total RNA was extracted from the cells. There are a total of 18 arrays with an n=3 per treatment group. Five treatment groups were used: 1) DMSO vehicle control at 4h; 2) 45 uM fluoranthene at 4h; 3) 40 uM total PAH mixture (20 uM 1-methylanthracene: 20 uM fluoranthene) at 4h; 4) 5 uM p38 MAPK inhibitor (SB203580) + 40 uM total PAH mixture (20 uM 1-methylanthracene: 20 uM fluoranthene) at 4h; 5) 40 uM total PAH mixture (20 uM 1-methylanthracene: 20 uM fluoranthene) at 8 h; 6) 5 uM p38 MAPK inhibitor (SB203580) + 40 uM total PAH mixture (20 uM 1-methylanthracene: 20 uM fluoranthene) at 8h.
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| Contributor(s) |
Osgood RS, Velmurugan K, Bushel PR, Xiong K, Xiong J, Upham BL, Bauer AK |
| Citation(s) |
28329830 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R15 ES024893 |
Understudied polycyclic aromatic hydrocarbons involved in pulmonary inflamation |
UNIVERSITY OF COLORADO DENVER |
ALISON K BAUER |
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| Submission date |
Jul 20, 2016 |
| Last update date |
Feb 21, 2018 |
| Contact name |
Alison Kendall Bauer |
| E-mail(s) |
alison.bauer@ucdenver.edu
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| Organization name |
University of Colorado Denver
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| Department |
Environmental and Occupational Health
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| Street address |
12850 E. Montview Blvd.; Rm 3125
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| City |
Aurora |
| State/province |
CO |
| ZIP/Postal code |
80045 |
| Country |
USA |
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| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA330728 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE84649_RAW.tar |
145.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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