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| Status |
Public on Jan 11, 2017 |
| Title |
Comparative transcriptome analysis of TCV-infected wildtype and dcl1-9 mutant Arabidopsis thaliana |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Belonging to the Carmovirus family, Turnip crinkle virus (TCV) is a positive-strand RNA virus that can infect Arabidopsis. Most Arabidopsis ecotypes are highly susceptible to TCV, except for the TCV resistant line Di-17 derived from ecotype Dijon. Previous studies showed that many of the stress related genes have changed significantly after TCV infection. Besides the virus-triggered genes, small RNAs also play critical roles in plant defense by triggering either transcriptional and/or post-transcriptional gene silencing. In this study, TCV-infected wildtype Arabidopsis thaliana and dcl1-9 mutant plants were subjected to transcriptome and small RNA analysis to investigate the role of DCL1 in virus defense network.
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| Overall design |
4 week old Arabidopsis thaliana ecotype Col-0 and dcl1-9 homozygous plants were inoculated by TCV. Leaves were collected at 7 dpi for RNA and sRNA extraction.
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| Contributor(s) |
Wu C, Wang SM |
| Citation(s) |
27782158 |
| |
| Submission date |
Aug 01, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Chao Wu |
| E-mail(s) |
wuchao@u.nus.edu
|
| Phone |
+6565168803
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| Organization name |
National University of Singapore
|
| Department |
Department of Biological Sciences
|
| Lab |
Prof. Wong Sek Man
|
| Street address |
21 Lower Kent Ridge Road
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| City |
Singapore |
| ZIP/Postal code |
119077 |
| Country |
Singapore |
| |
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| Platforms (1) |
| GPL13222 |
Illumina HiSeq 2000 (Arabidopsis thaliana) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE85070 |
Comparative mRNA and sRNA transcriptome analysis of TCV-infected wildtype and dcl1-9 mutant Arabidopsis thaliana |
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| Relations |
| BioProject |
PRJNA336058 |
| SRA |
SRP080774 |
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