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Status |
Public on Aug 05, 2016 |
Title |
Analysis of BES1-PIF4 interdependency in gene expression |
Organism |
Arabidopsis thaliana |
Experiment type |
Expression profiling by array
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Summary |
BES1 transcription factor is a master regulator of brassinosteroid signalling. Part of BES1 activity as a transcription factor is brought about upon dimerization with light-responsive PIF4 transcription factor. To analyse BES1-dependent transcriptional cascade, and to which extent BES1 requires PIF4/PIF5 for target gene regulation, we performed microarray analysis of the following phenotypes: Col-0, bes1-D, pif4pif5 and bes1-D;pif4pif5 in dark grown seedlings.
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Overall design |
Col-0, bes1-D, pif4pif5 and bes1-D;pif4pif5 5-days seedlings grown in the dark were used for RNA extraction. 3 independent biological samples were collected for each genoptye.
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Contributor(s) |
Prat S, Espinosa-Ruiz A, Martínez C |
Citation missing |
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Submission date |
Aug 04, 2016 |
Last update date |
Apr 19, 2017 |
Contact name |
Ana Espinosa-Ruiz |
E-mail(s) |
espinosa@cnb.csic.es
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Phone |
0034652402452
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Organization name |
CSIC
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Department |
Centro Nacional de Biotecnología
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Lab |
314
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Street address |
Darwin,3
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City |
Madrid |
State/province |
Madrid |
ZIP/Postal code |
28049 |
Country |
Spain |
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Platforms (1) |
GPL9020 |
Agilent-021169 Arabidopsis 4 Oligo Microarray (V4) (Feature Number version) |
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Samples (12)
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Relations |
BioProject |
PRJNA336559 |