|
| Status |
Public on Oct 05, 2017 |
| Title |
Transcriptome profiling of OsFBK1 rice transgenics |
| Platform organism |
Oryza sativa |
| Sample organism |
Oryza sativa Indica Group |
| Experiment type |
Expression profiling by array
|
| Summary |
Rice transgenic plants of the F-box encoding gene, OsFBK1, in the Pusa Basmati 1 (PB1) variety have been found to display differences in the anther and root phenotypes. In order to elucidate changes at the transcriptome level, microarray of the roots of 14-day-old seedlings of over-expresseion (OE) and knock-down (KD) lines along with vector control (VC) and wild type (WT) were carried out.
|
| |
|
| Overall design |
Seedlings of OsFBK1-OE, OsFBK1-KD, VC and WT grown for fourteen days at 28±1oC . RNA from the roots were extracted from each sample and hybridized on rice Affymetrix microarrays. Two biological replicates of each sample were used for microarray analysis. Overall, eight samples were analyzed representing the transgenic lines and wild type.
|
| |
|
| Contributor(s) |
Borah P, Khurana JP |
| Citation(s) |
29295941 |
| |
| Submission date |
Aug 19, 2016 |
| Last update date |
Jan 15, 2018 |
| Contact name |
Jitendra Paul Khurana |
| E-mail(s) |
khuranaj@genomeindia.org
|
| Phone |
91 11 24115126
|
| Organization name |
University of Delhi South Campus
|
| Department |
Department of Plant Molecular Biology
|
| Street address |
Benito Juarez Road
|
| City |
New Delhi |
| ZIP/Postal code |
110021 |
| Country |
India |
| |
|
| Platforms (1) |
| GPL2025 |
[Rice] Affymetrix Rice Genome Array |
|
| Samples (8)
|
| GSM2285098 |
Wild type Pusa Basmati-1 14-day-old seedling, Biological replicate 1 |
| GSM2285099 |
Wild type Pusa Basmati-1 14-day-old seedling, Biological replicate 2 |
| GSM2285100 |
Vector control 14-day old-seedling, Biological replicate 1 |
| GSM2285101 |
Vector control 14-day old-seedling, Biological replicate 2 |
| GSM2285102 |
OsFBK1 over-expression 14-day-old seedling, Biological replicate 1 |
| GSM2285103 |
OsFBK1 over-expression 14-day-old seedling, Biological replicate 2 |
| GSM2285104 |
OsFBK1 knock-down 14-day-old seedling, Biological replicate 1 |
| GSM2285105 |
OsFBK1 knock-down 14-day-old seedling, Biological replicate 2 |
|
| Relations |
| BioProject |
PRJNA339516 |
Less...
More...