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Series GSE86069 Query DataSets for GSE86069
Status Public on Aug 12, 2017
Title Dysregulation of a miR-23b/27b-p53 axis impairs muscle differentiation in humans with type 2 diabetes
Platform organisms Homo sapiens; Human alphaherpesvirus 1; Human alphaherpesvirus 2; Macacine alphaherpesvirus 1; Human betaherpesvirus 5; human gammaherpesvirus 4; Saimiriine gammaherpesvirus 2; JC polyomavirus; Human immunodeficiency virus 1; Human betaherpesvirus 6B; Human gammaherpesvirus 8; Merkel cell polyomavirus; Betapolyomavirus hominis; Betapolyomavirus macacae
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary Impaired skeletal muscle function is a central feature in the pathophysiology of type 2 diabetes (T2DM). The disease phenotype could be due to immature muscle cell development, which in turn may occur as the result of disturbed microRNA-mediated regulation of muscle differentiation in T2DM. To address this hypothesis, we assessed global miRNA expression during in vitro differentiation of muscle stem cells derived from T2DM patients and healthy controls. We identified the mir-23b/27b cluster to be downregulated in the patients, and further demonstrated that a pro-myogenic effect of these miRNAs occurs through targeting of several genes in the p53 pathway, which was concordantly dysregulated in the muscle cells derived from humans with T2DM. In conclusion, we have identified a novel myogenesis-controlling pathway, the miR-23b/27b-p53 axis, potentially contributing to the sustained multiple muscular dysfunctions in T2DM in humans.
 
Overall design Muscle stem cells were isolated from healthy donors (n=8) and donors with type 2 diabetes (n=8). RNA was harvested from proliferating myoblasts, during early myocyte differentiation and late myocyte differentiation.
 
Contributor(s) Henriksen TI, Davidsen PK, Pedersen M, Schultz HS, S Hansen N, Larsen TJ, Vaag A, Pedersen BK, Nielsen S, Scheele C
Citation(s) 28702332
Submission date Aug 25, 2016
Last update date Aug 15, 2017
Contact name Søren Nielsen
E-mail(s) soren_nielsen@inflammation-metabolism.dk
Organization name Rigshospitalet
Department Section 7641
Lab CFAS/CIM
Street address Blegdamsvej 9
City Copenhagen
State/province -
ZIP/Postal code 2100
Country Denmark
 
Platforms (1)
GPL22371 Exiqon, miRCURY LNA microRNA array, 6th gen (hsa, mirbase 17, Gene ID version)
Samples (47)
GSM2292900 Proliferating myocytes from healthy donor 1
GSM2292901 Early differentiating myocytes from healthy donor 1
GSM2292902 Late differentiating myocytes from healthy donor 1
Relations
BioProject PRJNA340166

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE86069_RAW.tar 98.7 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
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