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| Status |
Public on Nov 01, 2017 |
| Title |
miRNA expression data in unstimulated and TGF-β1-stimulated lung fibroblasts |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Lung fibroblasts play an important role in extracellular matrix homeostasis and this process is mainly regulated by transforming growth factor-beta (TGF-β). Hence, lung fibroblasts are postulated to play a crucial role in aberrant lung tissue repair and remodeling, which is a main factor in lung diseases like COPD. In this study, the effect of TGF-β1 on the miRNA expression in parenchymal lung fibroblasts is investigated.
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| Overall design |
Primary parenchymal lung fibroblasts of 9 control subjects were cultured. After serum starving for 24 hrs, the fibroblasts were stimulated with and without TGF-β1 (100 U/ml TGF-β1, equivalent to 3.1 ng/ml) for 24 hours. The RNA was isolated using TRIzol according to the protocol of the manufacturer. The RNA samples were then hybridized with the Human GeneChip miRNA 1.0 array (Affymetrix).
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| Contributor(s) |
Ong J, Timens W, Rajendran V, Algra A, Spira A, Lenburg ME, Campbell JD, van den Berge M, Postma DS, van den Berg A, Kluiver J, Brandsma C |
| Citation(s) |
28910321 |
| |
| Submission date |
Aug 29, 2016 |
| Last update date |
Nov 01, 2017 |
| Contact name |
Jennie Ong |
| E-mail(s) |
j.ong@umcg.nl
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| Organization name |
University Medical Center Groningen
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| Street address |
Hanzeplein 1
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| City |
Groningen |
| ZIP/Postal code |
9713 GZ |
| Country |
Netherlands |
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| Platforms (1) |
| GPL8786 |
[miRNA-1] Affymetrix Multispecies miRNA-1 Array |
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| Samples (18)
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| This SubSeries is part of SuperSeries: |
| GSE86186 |
miRNA expression data before and after TGF-β treatment and Ago2-associated transcripts in lung fibroblasts |
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| Relations |
| BioProject |
PRJNA340781 |
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