|
| Status |
Public on Nov 01, 2017 |
| Title |
Current smoker 5 |
| Sample type |
RNA |
| |
|
| Source name |
Primary parenchymal lung fibroblasts of a current smoking control subject
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: lung parenchyma
cell type: parenchymal lung fibroblasts
smoking status: Current smoker
|
| Treatment protocol |
After serum starving for 1 day, the fibroblasts were stimulated with 100 U/ml TGF-β for 24 hrs
|
| Growth protocol |
Primary lung fibroblasts were cultured with Ham's F12 containing 10% FCS. At passage 5, the fibroblasts were serum starved and subsequently stimulated with TGF-β
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
not provided.
|
| |
|
| Hybridization protocol |
not provided.
|
| Scan protocol |
not provided.
|
| Description |
Primary parenchymal lung fibroblasts, current smoker, untreated
|
| Data processing |
Robust Multi-array Average was performed using GeneSpring GX version 13.1.1 software for quantile normalization of the probe-level intensity measurements.
|
| |
|
| Submission date |
Aug 29, 2016 |
| Last update date |
Nov 01, 2017 |
| Contact name |
Jennie Ong |
| E-mail(s) |
j.ong@umcg.nl
|
| Organization name |
University Medical Center Groningen
|
| Street address |
Hanzeplein 1
|
| City |
Groningen |
| ZIP/Postal code |
9713 GZ |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL8786 |
| Series (2) |
| GSE86185 |
miRNA expression data in unstimulated and TGF-β1-stimulated lung fibroblasts |
| GSE86186 |
miRNA expression data before and after TGF-β treatment and Ago2-associated transcripts in lung fibroblasts |
|
