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| Status |
Public on May 01, 2017 |
| Title |
The histone demethylase KDM3A regulates the transcriptional program of the androgen receptor in prostate cancer cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To identify the genes regulated by androgen receptor (AR), we performed the profiling array analysis on the CWR22Rv1 cells and determined the differentially expressed genes upon the knockdown of AR.
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| Overall design |
CWR22Rv1 cells were transduced with the lentiviral particles (pLKO.1 control or AR shRNA) for 48 h. Total RNA was extracted using a Total RNA Miniprep Kit (Sigma). The knockdown efficiency of AR was confirmed by qRT-PCR analysis. RNA samples were sent to the Genomics Core Facility at University of Maryland for the quality check and processing for the Affymetrix Gene array analysis. Biological duplicate of samples were used in the study.
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| Contributor(s) |
Wilson S, Fan L, Sahgal N, Qi J, Filipp FV |
| Citation(s) |
28416760 |
| |
| Submission date |
Sep 07, 2016 |
| Last update date |
Mar 15, 2019 |
| Contact name |
Natasha Sahgal |
| Organization name |
University of Oxford
|
| Department |
Ludwig Institute of Cancer Research
|
| Street address |
Old Road Campus
|
| City |
Oxford |
| ZIP/Postal code |
OX3 7DQ |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA342775 |
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