|
| Status |
Public on May 01, 2017 |
| Title |
Rv1 pLKO.1_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
CWR22Rv1 prostate cancer cells expressing control pLKO.1 vector
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: CWR22Rv1 prostate cancer cells
genotye/variation: control
|
| Treatment protocol |
Cells were transduced with the lentiviral particles encoding control shRNA (pLKO.1) or AR shRNA (AR-KD) for 48 h.
|
| Growth protocol |
CWR22Rv1 cells were grown in the RPMI1640 media supplmentd with 10% FBS and antibiotics.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cells using the GenElute Mammalian Total RNA Purification Kit (Sigma).
|
| Label |
biotin
|
| Label protocol |
The samples were labeled, hybridized, wahsed and scanned by by the Biopolymer-Genomics Core Facility (Univeristy of Maryland School of Medicne)
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Affymetrix Gene Chip system 3000 7G
|
| Description |
replicate 1
|
| Data processing |
R and Bioconductor packages were used for processing and analyzing raw data to generate RMA normalized intensities
The dataset was filtered to retain only the main probesets followed by further excluding probes with low expression (<4).
|
| |
|
| Submission date |
Sep 07, 2016 |
| Last update date |
May 01, 2017 |
| Contact name |
Natasha Sahgal |
| Organization name |
University of Oxford
|
| Department |
Ludwig Institute of Cancer Research
|
| Street address |
Old Road Campus
|
| City |
Oxford |
| ZIP/Postal code |
OX3 7DQ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL16686 |
| Series (1) |
| GSE86547 |
The histone demethylase KDM3A regulates the transcriptional program of the androgen receptor in prostate cancer cells |
|
