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| Status |
Public on Nov 15, 2016 |
| Title |
Conversion of terminally committed mouse hepatocytes to culturable bipotent progenitor cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
A challenge for advancing approaches to liver regeneration is loss of functional differentiation capacity when hepatocyte progenitors are maintained in culture. Recent lineage-tracing studies have shown that mature hepatocytes (MHs) convert to an immature state during chronic liver injury, and we investigated whether this conversion could be recapitulated in vitro and if such converted cells could represent a source of expandable hepatocytes. We report that a cocktail of small molecules, Y-27632, A-83-01 and CHIR99021, can convert rat and mouse MHs in vitro into proliferative bipotent cells, which we term chemically induced liver progenitors (CLiPs). CLiPs can differentiate into both MHs and biliary epithelial cells that can form functional ductal structures. CLiPs in long-term culture did not lose their proliferative capacity or their hepatic differentiation ability, and rat CLiPs were shown to extensively repopulate chronically injured liver tissue. Thus our study advances the goals of liver regenerative medicine.
Transcriptomic analysis for mouse CLiPs at P1 which underwent hepatic induction (Matrix 1). Transcriptomic analysis for mouse CLiPs at P11-12 which underwent hepatic induction (Matrix 2).
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| Overall design |
Hepatic induction was performed for P1 mouse CLiPs or CLiPs that underwent 11-12 passages.
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| Contributor(s) |
Katsuda T, Ochiya T |
| Citation(s) |
27840021 |
| Submission date |
Oct 03, 2016 |
| Last update date |
Mar 22, 2017 |
| Contact name |
Takeshi Katsuda |
| E-mail(s) |
tkatsuda412@gmail.com
|
| Phone |
+1-215-746-5559
|
| Organization name |
University of Pennsylvania
|
| Department |
Perelman School of Medicine
|
| Lab |
Gastroenterology Division
|
| Street address |
421 Curie Blvd, BRB II/III Rm531
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21163 |
Agilent-074809 SurePrint G3 Mouse GE v2 8x60K Microarray [Probe Name version] |
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| Samples (21)
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| This SubSeries is part of SuperSeries: |
| GSE87764 |
Chemical induction of liver progenitor cells from mature hepatocytes |
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| Relations |
| BioProject |
PRJNA345376 |
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