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| Status |
Public on Oct 15, 2016 |
| Title |
Expression data from non-infected macrophages treated with conditioned medium from macrophages persistently infected with RSV virus |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Persistent Respiratory syncytial virus (RSV) infection in humans, animal models and cell lines have proven persistence of viral genomic RNA and mRNA for long periods of time. Cell lines are well-established models that can be used to study alterations in the expression profile of the host genome. We postulate that RSV persistence might also indirectly induce changes in the homeostasis of non-infected cells through soluble factors. Thus, we evaluated the effect of conditioned medium (CM) from MΦP cultures on the transcriptional profile of non-infected macrophages through gene expression microarrays
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| Overall design |
The persistently RSV infected macrophage-like cell line P388D1 (MΦP) was cultured during 24 and conditioned medium (CM-MΦP) was collected. Then, non-infected P388D1 macrophage-like cells (MΦN) were treated for 3 or 12 h with CM-MΦP or control-conditioned medium (CM-C) obtained from cultures of MΦN, passages 99 and 105.Total RNA was isolated and gene expression experiments were conducted per duplicate on the Affymetrix Mouse Gene 2.0 ST Array.
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| Contributor(s) |
Rivera-Toledo E, Salido-Guadarrama I, Rodríguez-Dorantes M, Torres-González L, Santiago-Olivares C, Gómez B |
| Citation(s) |
28069520 |
| |
| Submission date |
Oct 14, 2016 |
| Last update date |
Feb 21, 2018 |
| Contact name |
Ivan Salido-Guadarrama |
| E-mail(s) |
silvervann@gmail.com
|
| Phone |
52(55) 54871700
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| Organization name |
Instituto Nacional de Enfermedades Respiratorias
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| Lab |
Computational Biology
|
| Street address |
Calz. de Tlalpan 4502
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| City |
Mexico City |
| ZIP/Postal code |
14080 |
| Country |
Mexico |
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| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (8)
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| GSM2345003 |
CM-MΦP treatment at 3 hours, biological rep1 |
| GSM2345004 |
CM-MΦP treatment at 3 hours, biological rep2 |
| GSM2345005 |
CM-C treatment at 3 hours, biological rep1 |
| GSM2345006 |
CM-C treatment at 3 hours, biological rep2 |
| GSM2345007 |
CM-MΦP treatment at 12 hours, biological rep1 |
| GSM2345008 |
CM-MΦP treatment at 12 hours, biological rep2 |
| GSM2345009 |
CM-C treatment at 12 hours, biological rep1 |
| GSM2345010 |
CM-C treatment at 12 hours, biological rep2 |
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| Relations |
| BioProject |
PRJNA348440 |
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