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Series GSE89073 Query DataSets for GSE89073
Status Public on Mar 14, 2018
Title Expression data from WT and RGS5 KO mice treated with DOCA/salt
Organism Mus musculus
Experiment type Expression profiling by array
Summary The activation of vascular smooth muscle cells (VSMCs) during hypertension-induced arterial remodeling processes relies on a change of the gene expression program, i.e., up-regulation of genes to induce migration, proliferation and matrix degradation/synthesis. At the same time, genes controlling the quiescent, contractile VSMC phenotype are down-regulated. We used microarrays to detail the global program of gene expression underlying hypertension-induced vascular remodeling in the presence and absence of regulator of G-protein signaling 5 (RGS5) and identified distinct classes of down-regulated genes during vascular remodeling when RGS5 was not present.
 
Overall design WT and RGS5 KO mice (littermates) were treated with deoxycorticosterone acetate (DOCA; 50 mg/kg, 21-day-release pellets) and 1% NaCl in the drinking water for 10 days or left untreated. Afterwards, 2 femoral arteries per animal were isolated and RNA was extracted and hybridized on Affymetrix microarrays.
 
Contributor(s) Arnold C, Korff T
Citation(s) 29208700
Submission date Oct 23, 2016
Last update date Jun 13, 2018
Contact name Carsten Sticht
Organization name University Heidelberg
Department ZMF
Street address Theodor-Kutzer-Ufer
City Mannheim
ZIP/Postal code 68169
Country Germany
 
Platforms (1)
GPL22598 [MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array (mogene20st_Mm_ENTREZG_20.0.0)
Samples (12)
GSM2358408 WT mouse, biological rep. 1
GSM2358409 WT mouse, biological rep. 2
GSM2358410 WT mouse, biological rep. 3
Relations
BioProject PRJNA350226

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE89073_RAW.tar 116.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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