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Status |
Public on Mar 14, 2018 |
Title |
Expression data from WT and RGS5 KO mice treated with DOCA/salt |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
The activation of vascular smooth muscle cells (VSMCs) during hypertension-induced arterial remodeling processes relies on a change of the gene expression program, i.e., up-regulation of genes to induce migration, proliferation and matrix degradation/synthesis. At the same time, genes controlling the quiescent, contractile VSMC phenotype are down-regulated. We used microarrays to detail the global program of gene expression underlying hypertension-induced vascular remodeling in the presence and absence of regulator of G-protein signaling 5 (RGS5) and identified distinct classes of down-regulated genes during vascular remodeling when RGS5 was not present.
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Overall design |
WT and RGS5 KO mice (littermates) were treated with deoxycorticosterone acetate (DOCA; 50 mg/kg, 21-day-release pellets) and 1% NaCl in the drinking water for 10 days or left untreated. Afterwards, 2 femoral arteries per animal were isolated and RNA was extracted and hybridized on Affymetrix microarrays.
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Contributor(s) |
Arnold C, Korff T |
Citation(s) |
29208700 |
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Submission date |
Oct 23, 2016 |
Last update date |
Jun 13, 2018 |
Contact name |
Carsten Sticht |
Organization name |
University Heidelberg
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Department |
ZMF
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Street address |
Theodor-Kutzer-Ufer
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City |
Mannheim |
ZIP/Postal code |
68169 |
Country |
Germany |
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Platforms (1) |
GPL22598 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array (mogene20st_Mm_ENTREZG_20.0.0) |
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Samples (12)
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GSM2358411 |
WT mouse with hypertension, biological rep. 1 |
GSM2358412 |
WT mouse with hypertension, biological rep. 2 |
GSM2358413 |
WT mouse with hypertension, biological rep. 3 |
GSM2358414 |
RGS5 KO mouse, biological rep. 1 |
GSM2358415 |
RGS5 KO mouse, biological rep. 2 |
GSM2358416 |
RGS5 KO mouse, biological rep. 3 |
GSM2358417 |
RGS5 KO mouse with hypertension, biological rep. 1 |
GSM2358418 |
RGS5 KO mouse with hypertension, biological rep. 2 |
GSM2358419 |
RGS5 KO mouse with hypertension, biological rep. 3 |
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Relations |
BioProject |
PRJNA350226 |
Supplementary file |
Size |
Download |
File type/resource |
GSE89073_RAW.tar |
116.3 Mb |
(http)(custom) |
TAR (of CEL) |
Processed data included within Sample table |
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