|
| Status |
Public on Mar 14, 2018 |
| Title |
WT mouse, biological rep. 3 |
| Sample type |
RNA |
| |
|
| Source name |
WT mouse untreated
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57BL/6
genotype/variation: WT
gender: male
tissue: femoral arteries
treatment: none
hypertension: Ctrl
|
| Treatment protocol |
At least 12-week-old male wild type C57BL/6J or RGS-deficient mice were anesthetized with 1.75% isoflurane/0.8 L/min room air and hypertension was induced by subcutaneously implanting slow-release DOCA pellets (M-121, 21-day-release, 50 mg/kg, Innovative Research of America, Sarasota, FL) according to the manufacturer’s instructions and feeding the mice with 1% NaCl in the drinking water. The animals were euthanized after 10 days after in situ perfusion of the arterial system with ice-cold PBS. Femoral arteries were isolated and cleared from any surrounding tissue and denuded by cannulation. The arteries were then collected in RNAlater (Qiagen) and frozen in Trizol.
|
| Growth protocol |
Animal studies were performed with permission of the Regional Council Karlsruhe and conformed to the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health (NIH Publication No. 85-23, revised 1996). The rgs5GFP/GFP mouse strain (genetic background: F1H4 ((129S6/SvEvTac x C57BL/6NTac)F1) * C57BL/6) was backcrossed >15 generations onto the C57BL/6 background.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
|
| Scan protocol |
Affymetrix GeneArray Scanner3000.
|
| Description |
WT_Ctrl_19
Gene expression data of femoral arteries of untreated WT mice.
|
| Data processing |
The data were analyzed with a commercial software called JMP Genomics, version 6, from SAS. Gene expression profiling was performed using arrays of mouse MoGene-2_0-type from Affymetrix. A Custom CDF Version 20 with Entrez Gene-based gene definitions was used to annotate the arrays. The raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Median polish Probeset Summary.
|
| |
|
| Submission date |
Oct 23, 2016 |
| Last update date |
Mar 14, 2018 |
| Contact name |
Carsten Sticht |
| Organization name |
University Heidelberg
|
| Department |
ZMF
|
| Street address |
Theodor-Kutzer-Ufer
|
| City |
Mannheim |
| ZIP/Postal code |
68169 |
| Country |
Germany |
| |
|
| Platform ID |
GPL22598 |
| Series (1) |
| GSE89073 |
Expression data from WT and RGS5 KO mice treated with DOCA/salt |
|
