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Series GSE89399 Query DataSets for GSE89399
Status Public on Dec 13, 2016
Title Clonal variation in drug and radiation response among glioma-initiating cells is linked to proneural-mesenchymal transition (HTA 2.0)
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Intra-tumor heterogeneity is a hallmark of glioblastoma multiforme, and thought to negatively affect treatment efficacy. Here we establish libraries of glioma-initiating cell (GIC) clones from patient samples and find extensive molecular and phenotypic variability between clones, including a wide range of responses to radiation and drugs. This widespread variability was observed as a continuum of multitherapy resistance phenotypes linked to a proneural-to-mesenchymal shift in the transcriptome.
 
Overall design Affymetrix HTA 2.0 array analyses were performed according to the manufacturer's directions on total RNA extracted from 115 clonal malignant glioma cultures and 6 bulk cultures representing the parental material used to derive clones. 25 bilogical replicates were also included.
 
Contributor(s) Segerman B, Westermark B
Citation(s) 27974212
Submission date Nov 01, 2016
Last update date Oct 29, 2018
Contact name Bo Segerman
E-mail(s) bo.segerman@igp.uu.se
Organization name Uppsala University
Street address Dag Hammarskjolds v 20
City Uppsala
ZIP/Postal code 751 85
Country Sweden
 
Platforms (1)
GPL17586 [HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version]
Samples (146)
GSM2368489 Malignant glioma clone U3017MG-C56
GSM2368490 Malignant glioma clone U3017MG-C64
GSM2368491 Malignant glioma clone U3017MG-C70
This SubSeries is part of SuperSeries:
GSE89401 Clonal variation in drug and radiation response among glioma-initiating cells is linked to proneural-mesenchymal transition
Relations
BioProject PRJNA352067

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE89399_RAW.tar 3.5 Gb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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