|
| Status |
Public on Dec 13, 2016 |
| Title |
Malignant glioma clone U3017MG-C5 |
| Sample type |
RNA |
| |
|
| Source name |
Malignant glioma clone U3017MG-C5
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Malignant glioma clone
cell line: U3017MG-C5
|
| Growth protocol |
adherent cultures on laminin-coated dishes using neural stem cell media
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Plus Mini Kit, QIAGEN
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 250 ng total RNA by using GeneChip® WT PLUS Reagent Kit
|
| |
|
| Hybridization protocol |
GeneChip® Human Transcriptome Array 2.0 were hybridized for 16 hours in a 45°C incubator. According to the GeneChip® Expression Wash, Stain and Scan Manual (PN 702731 Rev. 3, Affymetrix Inc., Santa Clara, CA) the arrays were washed and stained using the Fluidics Station 450
|
| Scan protocol |
scanned using the GeneChip® Scanner 3000 7G
|
| Data processing |
The data were analyzed with Affymetrix® Expression Console™ Software using Affymetrix default analysis settings with the Robust Multiarray Averaging (RMA) algorithm.
HTA-2_0.r3.pgf
HTA-2_0.r3.Psrs.mps
|
| |
|
| Submission date |
Nov 01, 2016 |
| Last update date |
Dec 13, 2016 |
| Contact name |
Bo Segerman |
| E-mail(s) |
bo.segerman@igp.uu.se
|
| Organization name |
Uppsala University
|
| Street address |
Dag Hammarskjolds v 20
|
| City |
Uppsala |
| ZIP/Postal code |
751 85 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL17586 |
| Series (2) |
| GSE89399 |
Clonal variation in drug and radiation response among glioma-initiating cells is linked to proneural-mesenchymal transition (HTA 2.0) |
| GSE89401 |
Clonal variation in drug and radiation response among glioma-initiating cells is linked to proneural-mesenchymal transition |
|
