|
| Status |
Public on Dec 05, 2007 |
| Title |
Identification of Myc-regulated microRNAs in human P493 cells |
| Platform organisms |
Caenorhabditis elegans; Homo sapiens; Human betaherpesvirus 5; human gammaherpesvirus 4; Murid gammaherpesvirus 4; Human gammaherpesvirus 8 |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
We previously described the use of a spotted oligonucleotide array to identify the mir-17 cluster as a direct transcriptional target of Myc. In order to determine whether Myc regulates additional miRNAs, we produced custom microarrays with an expanded set of probes capable of assaying the expression of 313 human miRNAs and 233 mouse miRNAs. P493-6 cells which are Epstein-Barr virus-immortalized human B cells that harbor a tetracycline (tet)-repressible allele of Myc were studied. These cells are tumorigenic in immunocompromised mice and represent a model of human B cell lymphoma. miRNA expression profiles were examined in the high Myc (-tet) and low Myc (+tet) state.
Keywords: Dose response
|
| |
|
| Overall design |
P493 cells with high Myc (-tet) and low Myc (+tet) were compared.
|
| |
|
| Contributor(s) |
Chang T, Yu D, Arking DE, West KM, Thomas-Tikhonenko A, Mendell JT |
| Citation(s) |
18066065 |
| |
| Submission date |
Sep 17, 2007 |
| Last update date |
Mar 19, 2012 |
| Contact name |
Tsung-Cheng Chang |
| Organization name |
Johns Hopkins University School of Medicine
|
| Street address |
733 N. Broadway
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL5847 |
Human microRNA 1K array version 1.0 |
|
| Samples (2) |
|
| This SubSeries is part of SuperSeries: |
| GSE9129 |
Identification of Myc-regulated microRNAs: human and mouse |
|
| Relations |
| BioProject |
PRJNA105209 |
