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| Status |
Public on Dec 31, 2017 |
| Title |
Mechanism of early light signaling by the carboxy-terminal output module of Arabidopsis phytochrome B |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Phytochromes are evolutionarily conserved photoreceptors in bacteria, fungi, and plants. The prototypical phytochrome comprises an N-terminal photosensory module and a C-terminal histidine kinase signaling-output module. However, the plant phytochrome has been postulated to transduce light signals by interacting with a group of nodal Phytochrome-Interacting transcription Factors (PIFs) and triggering their degradation via the N-terminal photosensory module, while its C-terminal output module, including a Histidine Kinase-Related Domain (HKRD), is thought not to participate directly in signaling. Here, we show that the C-terminal module of Arabidopsis phytochrome B (PHYB) is unexpectedly sufficient to mediate the degradation of PIF3 and to induce a distinct set of PIF-regulated photosynthetic genes. These signaling functions require the HKRD and particularly its dimerization. A D1040V mutation, which disrupts the dimerization of HKRD and the interaction between the C-terminal module and PIF3, abrogates the early light signaling functions of PHYB in nuclear accumulation, photobody biogenesis, and PIF3 degradation. In contrast, disruption of the interaction between PIF3 and PHYB’s N-terminal photosensory module has little effect on PIF3 degradation. Together, this study provides novel insight into the central mechanism of early phytochrome signaling that the C-terminal signaling-output module of PHYB interacts with PIF3 in the nucleus to mediate PIF3 degradation by light.
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| Overall design |
Whole seedling mRNA profiles of 100h dark-grown phyB-9 mutant and BCY overexpression line were generated by deep sequencing, in triplicate, using Illumina NextSeq 500
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| Contributor(s) |
Qiu Y, Pasoreck EK, Long L, Reddy AK, Nagatani A, Ma W, Chory J, Chen M |
| Citation(s) |
29199270 |
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| Submission date |
Dec 06, 2016 |
| Last update date |
Jul 25, 2024 |
| Contact name |
Meng Chen |
| E-mail(s) |
meng.chen@ucr.edu
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| Phone |
9518275635
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| Organization name |
University of California, Riverside
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| Street address |
2150 Batchelor Hall, 900 University Ave.
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| City |
Riverside |
| State/province |
CA |
| ZIP/Postal code |
92521 |
| Country |
USA |
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| Platforms (1) |
| GPL19580 |
Illumina NextSeq 500 (Arabidopsis thaliana) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA356373 |
| SRA |
SRP094618 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE90925_gene_exp.diff.gz |
1012.0 Kb |
(ftp)(http) |
DIFF |
SRA Run Selector |
| Processed data are available on Series record |
| Raw data are available in SRA |
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