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Series GSE9144 Query DataSets for GSE9144
Status Public on Nov 14, 2007
Title Spontaneous SCC induced by the somatic inactivation of Rb1 and Trp53 tumor suppressors in mouse stratified epithelia
Organism Mus musculus
Experiment type Expression profiling by array
Summary The squamous cell carcinomas represent the aggressive type of non melanoma skin cancer, the most frequent malignancy among human population. We have studied here the possible relationship between these two pathways in skin using epidermal-specific mutant mice. Loss of p53, but not pRb, produces spontaneous tumor development, indicating that, contrary to pRb, p53 is the predominant tumor suppressor acting in mouse epidermis. The simultaneous inactivation of pRb and p53 does not aggravate the epidermal phenotype observed in Rb-deficient mice in terms of proliferation and/or differentiation. However, in doubly deficient mice spontaneous skin tumor development is severely accelerated. The tumors are aggressive, undifferentiated and display a hair follicle origin. Detailed analysis indicates that the acceleration is mediated by premature activation of the EGFR/Akt pathway, resulting in increased angiogenesis. The molecular characteristics of this model provide valuable tools to understand epidermal tumor formation, and may ultimately contribute to the development of therapies for the treatment of aggressive squamous cancer.
Keywords: Squamous cell carcinoma, Epidermis, Tumorigenesis, pRb, p53, Akt, Angiogenesis
 
Overall design Pools from RNA whole skin extracts from 3 animals of same genotype were done and analyzed, per duplicate, in mouse microarrays. Comparison was performed between the 4 different genotypes.
 
Contributor(s) Martínez-Cruz AB, Santos M, Lara MF, Segrelles C, Ruiz S, Moral M, Lorz C, García-Escudero R, Paramio JM
Citation(s) 18245467
Submission date Sep 24, 2007
Last update date Jan 08, 2019
Contact name Ramon Garcia-Escudero
E-mail(s) ramon.garcia@ciemat.es, rgescudero@gmail.com
Organization name CIEMAT
Department Biomedical Innovation
Lab Molecular and Traslational Oncology
Street address Avda Complutense 40, Ed 70A
City Madrid
State/province Madrid
ZIP/Postal code 28040
Country Spain
 
Platforms (1)
GPL339 [MOE430A] Affymetrix Mouse Expression 430A Array
Samples (8)
GSM231132 Control1
GSM231133 Control2
GSM231140 pRb-/-;p53+/+.1
Relations
BioProject PRJNA102687

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE9144_RAW.tar 27.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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