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| Status |
Public on Apr 29, 2008 |
| Title |
Human GBM tumor vs Normal Human DNA |
| Organism |
Homo sapiens |
| Experiment type |
Genome variation profiling by genome tiling array
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| Summary |
We have developed a nonheuristic genome topography scan (GTS) algorithm to characterize the patterns of genomic alterations in human glioblastoma (GBM), identifying frequent p18INK4C and p16INK4A codeletion. Functional reconstitution of p18INK4C in GBM cells null for both p16INK4A and p18INK4C resulted in impaired cell-cycle progression and tumorigenic potential. Conversely, RNAi-mediated depletion of p18INK4C in p16INK4A-deficient primary astrocytes or established GBM cells enhanced tumorigenicity in vitro and in vivo. Furthermore, acute suppression of p16INK4A in primary astrocytes induced a concomitant increase in p18INK4C. Together, these findings uncover a feedback regulatory circuit in the astrocytic lineage and demonstrate a bona fide tumor suppressor role for p18INK4C in human GBM wherein it functions cooperatively with other INK4 family members to constrain inappropriate proliferation.
Keywords: comparative genomic hybridization
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| Overall design |
DNA copy number abberation of human glioblastoma tumors were obtained by comparative genomic hybridization of GBM tumor vs. normal human DNA. 11 human GBM samples were analyzed on Agilent human 244A human cgh array (G4411B). Normal Human DNA was used as reference. Some samples were hybridized with dye-swap replica.
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| Contributor(s) |
Wiedemeyer R, Brennan C, Heffernan TP, Xiao Y, Mahoney J, Protopopov A, Zheng H, Bignell G, Furnari F, Cavenee WK, Hahn WC, Ichimura K, Collins VP, Chu GC, Stratton MR, Ligon KL, Futreal PA, Chin L |
| Citation(s) |
18394558 |
| |
| Submission date |
Sep 26, 2007 |
| Last update date |
Oct 26, 2016 |
| Contact name |
Yonghong Xiao |
| E-mail(s) |
yonghong_xiao@yahoo.com
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| Organization name |
Dana-Farber Cancer Institute
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| Street address |
450 Brookline Ave., HIM218A
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL4091 |
Agilent-014693 Human Genome CGH Microarray 244A (Feature number version) |
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| Samples (11)
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| This SubSeries is part of SuperSeries: |
| GSE9200 |
Feedback Circuit among INK4 Tumor Suppressors Constrains Human Glioblastoma Development |
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| Relations |
| BioProject |
PRJNA105241 |
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