|
| Status |
Public on Apr 29, 2008 |
| Title |
Glioblastoma GBM_183_acgh |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
human glioblastoma tumor
|
| Organism |
Homo sapiens |
| Characteristics |
Human glioblastoma tumor
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Total DNA extracted using PureGene kit following manufacturer's instructions (Gentra Systems).
|
| Label |
Cy3
|
| Label protocol |
Genomic DNA Labeling Kit (Enzo Life Sciences, Farmingdale, NY) was used as per manufacturer's protocol.
|
| |
|
| Channel 2 |
| Source name |
universal human male genomic DNA
|
| Organism |
Homo sapiens |
| Characteristics |
normal human male genomic DNA
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Total DNA extracted using PureGene kit following manufacturer's instructions (Gentra Systems).
|
| Label |
Cy5
|
| Label protocol |
Genomic DNA Labeling Kit (Enzo Life Sciences, Farmingdale, NY) was used as per manufacturer's protocol.
|
| |
|
| |
| Hybridization protocol |
Genomic DNA hybridization to Agilent CGH arrays were performed as per manufacturer’s protocol
|
| Scan protocol |
Scanning was performed as per manufacturer's protocol
|
| Description |
comparative genomic hybridization of human glioblastoma vs. normal genomic DNA
|
| Data processing |
LOWESS normalized, background subtracted data obtained from log2 of processed Red signal/processed Green signal. Agilent software was used.
|
| |
|
| Submission date |
Sep 26, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Yonghong Xiao |
| E-mail(s) |
yonghong_xiao@yahoo.com
|
| Organization name |
Dana-Farber Cancer Institute
|
| Street address |
450 Brookline Ave., HIM218A
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL4091 |
| Series (2) |
| GSE9177 |
Human GBM tumor vs Normal Human DNA |
| GSE9200 |
Feedback Circuit among INK4 Tumor Suppressors Constrains Human Glioblastoma Development |
|
