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| Status |
Public on Dec 13, 2016 |
| Title |
miRNA-seq of lung in two kinds of pig breeds after PCV2 injection |
| Organism |
Sus scrofa |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Postweaning multisystemic wasting syndrome in pigs has devastated the swine industry since the 1990s. Porcine circovirus type 2 (PCV2) is the primary cause of this disease. MicroRNAs (miRNAs) are small noncoding RNAs that play important roles in regulating gene expression, especially at the post-transcription level. The expression profiles of miRNAs have been reported in porcine reproductive and respiratory syndrome, porcine parvovirus, and other pig diseases; however, the miRNA expression profiles in pigs infected with PCV2 have not been reported so far. The Laiwu pig (a Chinese indigenous pig breed) has different meat quality, adipogenesis, and disease-resistance from western commercial pig breeds. In this study, four small RNA libraries were constructed from the lung tissue of uninfected and infected Laiwu and Yorkshire/Landrace crossbred (YL) pigs. High-throughput sequencing and bioinformatics were used to determine the abundance and differential expression of miRNAs in the four libraries
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| Overall design |
miRNA-seq of four groups as PCV2 infected Laiwu pig (Lw-i), uninfected Laiwu pig (Lw-un), infected western commercial (YL-i) and uninfected western commercial pigs (YL-un)
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| Contributor(s) |
Wang L |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 12, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Liyuan Wang |
| E-mail(s) |
651312661@qq.com
|
| Phone |
+8618518153609
|
| Organization name |
China Agricultura University
|
| Department |
Animal Sciencie and Technology
|
| Lab |
Lab of Animal Genetic Resourse and Molecular Breeding
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| Street address |
No.2 of Yuanmingyuan West Road, Haidian District
|
| City |
Beijing |
| State/province |
China |
| ZIP/Postal code |
+86-100094 |
| Country |
China |
| |
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| Platforms (1) |
| GPL11429 |
Illumina HiSeq 2000 (Sus scrofa) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA357045 |
| SRA |
SRP094946 |
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