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| Status |
Public on Jun 01, 2017 |
| Title |
Comparative toxicogenomic effects of three IMX-101 constituents DNAN, NTO and NQ on C. elegans |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by array
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| Summary |
We used the soil nematode Caenorhabditis elegans, a well-characterized genomics model organism, as the test organism and a species-specific, genome-wide 44K-oligo probe microarray for gene expression analysis. Our goal was to discern toxicological mechanisms for three insensitive munitions formulation IMX-101 constituents 2,4-dinitroanisole (DNAN), nitroguanidine (NQ) and nitrotriazolone (NTO) at the molecular level.
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| Overall design |
Gene expression profiling was performed immediately after the 24-hr exposure for each chemical at the lowest, medium and highest sublethal concentrations plus the control with four replicates per treatment. Six 8 x 60K formatted slides (each 60K array containing 44K control and non-control probes, i.e., 27% spots empty) were used. Total RNA samples were randomized when hybridized to the arrays.
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| Contributor(s) |
Gong P, Donohue KB, Mayo AM, Wilbanks MS, Barker ND, Guan X, Gust KA |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 13, 2016 |
| Last update date |
Jun 03, 2017 |
| Contact name |
Ping Gong |
| E-mail(s) |
ping.gong@usace.army.mil
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| Phone |
(601) 634-3521
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| Organization name |
U.S Army Engineer Research and Development Center
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| Department |
Environmental Laboratory
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| Street address |
3909 Halls Ferry Road
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| City |
Vicksburg |
| State/province |
Mississippi |
| ZIP/Postal code |
39180 |
| Country |
USA |
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| Platforms (1) |
| GPL22795 |
[Agilent-064832] C. elegans gene expression 8x44K olig array |
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| Samples (48)
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| Relations |
| BioProject |
PRJNA357354 |
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