|
| Status |
Public on Dec 27, 2017 |
| Title |
Transcriptome analysis of Agrobacterium sp. ATCC 31749 wild strain and ΔfnrN based on RNA-seq |
| Organism |
Agrobacterium sp. ATCC 31749 |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose:first,we want to find the genes revelant to curdlan synthesis and oxygen regulation, second, we want to research the function of fnrN gene in Agrobacterium sp. ATCC 31749. Method: samples of cell growth phase, curdlan-producing phase (normoxia) and curdlan-producing phase (micro-oxygen treated) in both Agrobacterium sp. ATCC 31749 wild strain and ΔfnrN strain were collectecd to extract mRNA. Each sample was treated in duplicate. The softwares we used include fastqc, trimmomatic, TopHat2 and Cufflinks. Illumina Hiseq4000 was used to complete the research.
|
| |
|
| Overall design |
12 samples: samples of cell growth phase, curdlan-producing phase (normoxia) and curdlan-producing phase (micro-oxygen treated) in both Agrobacterium sp. ATCC 31749 wild strain and ΔfnrN strain were collectecd to extract mRNA. Each sample was treated in duplicate.
|
| |
|
| Contributor(s) |
Zheng Z, Wang Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 27, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
王 永远 |
| E-mail(s) |
529974917@qq.com
|
| Phone |
13771099543
|
| Organization name |
江南大学
|
| Street address |
滨湖区蠡湖街道1800号
|
| City |
无锡 |
| State/province |
江苏省 |
| ZIP/Postal code |
214122 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL22859 |
Illumina HiSeq 4000 (Agrobacterium sp. ATCC 31749) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA359112 |
| SRA |
SRP095704 |
Less...
More...