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Status |
Public on Oct 01, 2011 |
Title |
Age Dependent Decrease in Vascular Remodeling Response in LDLr-/- mice unveils a role of Quaking in Vascular Homeostasis |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
Female C57Bl/6J LDLr -/- mice, aged 12 weeks (n=16) and 52 weeks (n=14), were put on western-type diet for 6 weeks before perivascular bilateral collar placement in the carotid artery. RNA was isolated from the carotid artery 6 weeks after collar placement. Snap frozen carotid arteries were pooled in four subgroups of three samples per age group in order to yield a sufficient amounts of RNA. Gene expression profiles from young and old mice were compared on spotted oligonucleotide arrays containing the 22K mouse Sigma-Compugen libarary v1. A direct hybridization was used with dye swaps for each pool. Keywords: RNA expression profiling
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Overall design |
Four pools of each strain were used. Direct hybridization design. Dye swap for each pair of samples. In total 8 arrays.
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Contributor(s) |
't Hoen PA, Kraaijeveld AO, van der Lans CA, Biessen EA |
Citation missing |
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Submission date |
Oct 12, 2007 |
Last update date |
Mar 17, 2012 |
Contact name |
Peter A.C. 't Hoen |
E-mail(s) |
p.a.c.hoen@lumc.nl
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Phone |
+31 71 5269421
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Fax |
+31 71 5268285
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URL |
http://www.humgen.nl
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Organization name |
Leiden University Medical Center
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Department |
Center for Human and Clinical Genetics
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Street address |
PO Box 9600
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City |
Leiden |
ZIP/Postal code |
2300 RC |
Country |
Netherlands |
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Platforms (1) |
GPL5964 |
LGTC_Mouse_SigmaCompugen_Mu22K_Rosetta |
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Samples (8)
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Relations |
BioProject |
PRJNA102965 |