|
| Status |
Public on Oct 01, 2011 |
| Title |
carotid_artery-pair3b_CL51 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Old_pool3
|
| Organism |
Mus musculus |
| Characteristics |
Female C57Bl/6J LDLr -/- mice, aged 64 weeks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Carotid samples were homogenized in Guanidium thiocyanate-phenol. After RNA extraction, RNA was further purified using an RNeasy mini column (Qiagen, Venlo, the Netherlands).
|
| Label |
Cy3
|
| Label protocol |
Between 130 and 500 ng of total RNA was amplified one time according to the Eberwine procedure by the Ambion MessageAmp kit (Ambion, Austin, TX). Aminoallyl UTPs (A5660, Sigma Diagnostics) were incorporated during in vitro transcription. 3 μicrograms of aminoallyl-modified nucleotides were coupled with monoreactive Cy3 and Cy5 dyes (Amersham, Piscataway, NJ).
|
| |
|
| Channel 2 |
| Source name |
Young_pool3
|
| Organism |
Mus musculus |
| Characteristics |
Female C57Bl/6J LDLr -/- mice, aged 24 weeks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Carotid samples were homogenized in Guanidium thiocyanate-phenol. After RNA extraction, RNA was further purified using an RNeasy mini column (Qiagen, Venlo, the Netherlands).
|
| Label |
Cy5
|
| Label protocol |
Between 130 and 500 ng of total RNA was amplified one time according to the Eberwine procedure by the Ambion MessageAmp kit (Ambion, Austin, TX). Aminoallyl UTPs (A5660, Sigma Diagnostics) were incorporated during in vitro transcription. 3 μicrograms of aminoallyl-modified nucleotides were coupled with monoreactive Cy3 and Cy5 dyes (Amersham, Piscataway, NJ).
|
| |
|
| |
| Hybridization protocol |
Oligonucleotide micro array slides were hybridized overnight at 55C in a GeneTAC hybridization station (Genomic Solutions, Ann Arbor, MI).
|
| Scan protocol |
Slides were scanned in an Agilent G2565BA microarray scanner at 30% PMT power
|
| Description |
Gene expression profiles from young and old mice were compared
|
| Data processing |
Feature extraction was done with GenePix 4.0. Data were processed using Rosetta's standard error model for GenePix
VALUE column represents normalized, log10-transformed (young/old) data
|
| |
|
| Submission date |
Oct 12, 2007 |
| Last update date |
Oct 01, 2011 |
| Contact name |
Peter A.C. 't Hoen |
| E-mail(s) |
p.a.c.hoen@lumc.nl
|
| Phone |
+31 71 5269421
|
| Fax |
+31 71 5268285
|
| URL |
http://www.humgen.nl
|
| Organization name |
Leiden University Medical Center
|
| Department |
Center for Human and Clinical Genetics
|
| Street address |
PO Box 9600
|
| City |
Leiden |
| ZIP/Postal code |
2300 RC |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL5964 |
| Series (1) |
| GSE9310 |
Age Dependent Decrease in Vascular Remodeling Response in LDLr-/- mice unveils a role of Quaking in Vascular Homeostasis |
|
