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| Status |
Public on Jan 06, 2017 |
| Title |
IgH Isotype-Specific B Cell Receptor Dosage Regulates B Cell Fate |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We use polyclonal Ighγ1/γ1 and Ighε/ε mice (which initially produce only IgG1 or IgE from their respective native genomic configurations) to examine the role of IgH isotype on BCR function. We use microarray expression analysis to compare the internal state of the three IgH isotype specific B-cells. Microarray was used to compare the expression level of various genes in splenic follicular B-cells of Ighε/ε, Ighγ1/γ1 and Ighwt mice. Each mouse strain also had a naturally pre-assembled Ig light chain (VJk5).
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| Overall design |
The follicular B-cells were sorted form spleen of polyclonal Ighε/ε, Ighγ1/γ1 and Ighwt mice. RNA extracted from trizol was used for the analysis. 3 independent replicates were performed.
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| Contributor(s) |
Tong P, Granato A, Zuo T, Chaudhary N, Han SS, Donthula R, Shrestha A, Sen D, Wesemann DR |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jan 05, 2017 |
| Last update date |
Feb 21, 2018 |
| Contact name |
Duane R. Wesemann |
| E-mail(s) |
DWESEMANN@BWH.HARVARD.EDU
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| Organization name |
Brigham and Women's Hospital
|
| Department |
Medicine, Division of Allergy and Clinical Immunology
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| Street address |
77 Avenue Louis Pasteur
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (9)
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| GSM2448630 |
Igh[epsilon]/[epsilon]-Spleenic Follicular B-cells rep1 |
| GSM2448631 |
Igh[epsilon]/[epsilon]-Spleenic Follicular B-cells rep2 |
| GSM2448632 |
Igh[epsilon]/[epsilon]-Spleenic Follicular B-cells rep3 |
| GSM2448633 |
Igh[gamma]1/[gamma]1-Spleenic Follicular B-cells rep1 |
| GSM2448634 |
Igh[gamma]1/[gamma]1-Spleenic Follicular B-cells rep2 |
| GSM2448635 |
Igh[gamma]1/[gamma]1-Spleenic Follicular B-cells rep3 |
| GSM2448636 |
Ighwt-Spleenic Follicular B-cells rep1 |
| GSM2448637 |
Ighwt-Spleenic Follicular B-cells rep2 |
| GSM2448638 |
Ighwt-Spleenic Follicular B-cells rep3 |
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| Relations |
| BioProject |
PRJNA360298 |
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