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Series GSE93433 Query DataSets for GSE93433
Status Public on Jun 22, 2017
Title The histone acetyltransferase Mst2 prevents epigenetic silencing via specific acetylation of the ubiquitin ligase Brl1 [microarray data]
Organism Schizosaccharomyces pombe
Experiment type Genome variation profiling by genome tiling array
Summary Faithful propagation of distinct chromatin states is crucial to maintain cellular identity. Whereas mechanisms that sustain repressed states have been intensely studied, regulatory circuits that protect active chromatin from inactivating signals are not well understood. Here we report the discovery of a self-reinforcing feedback loop that preserves the transcription-competent state of RNA polymerase II transcribed genes. We found that the chromatin reader Pdp3 recruits the histone acetyltransferase Mst2 to H3K36me3 marked chromatin. Thereby, Mst2 binds to all transcriptionally active regions genome-wide. Besides histone H3K14, Mst2 specifically acetylates Brl1, a component of the histone H2B ubiquitin ligase complex (HULC). Brl1 acetylation results in increased histone H2B ubiquitination, which together with H3K14ac positively feeds back on transcription and prevents assembly of ectopic heterochromatin. Our work uncovers a molecular pathway that secures epigenome integrity and highlights the importance of opposing feedback loops for the partitioning of chromatin into transcriptionally active and inactive states.
Overall design The study comprises 18 datasets from cells expressing a Dam fusion construct at low levels: 5 control Dam-only strains, 4 Dam-Mst2 in wild type cells, 5 Dam-Mst2 in pdp3D cells, 2 Dam-Mst2 in set2D cells, 2 Dam-Mst2 in set1D cells
Contributor(s) Flury V, Georgescu PR, Iesmantavicius V, Shimada Y, Kuzdere T, Braun S, Bühler M
Citation(s) 28648780
Submission date Jan 11, 2017
Last update date Sep 21, 2017
Contact name Fabio Mohn
Organization name Friedrich Miescher Institute for Biomedical Research
Lab Buehler Lab
Street address Maulbeerstrasse 66
City Basel
ZIP/Postal code 4058
Country Switzerland
Platforms (1)
GPL7715 [Sp20b_M] Affymetrix S. pombe Tiling 1.0FR Array
Samples (18)
GSM2453311 Damonly_492.1
GSM2453312 Damonly_492.2
GSM2453313 Damonly_spb492_1
This SubSeries is part of SuperSeries:
GSE93434 The histone acetyltransferase Mst2 prevents epigenetic silencing via specific acetylation of the ubiquitin ligase Brl1
BioProject PRJNA361033

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE93433_DamID_results.txt.gz 2.2 Mb (ftp)(http) TXT
GSE93433_RAW.tar 202.5 Mb (http)(custom) TAR (of CEL)
GSE93433_sPombechromosomalregionsV02.txt.gz 356 b (ftp)(http) TXT
GSE93433_sPombegenomicelementsV02_ALL.txt.gz 305.2 Kb (ftp)(http) TXT
Processed data are available on Series record

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