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| Status |
Public on Jun 22, 2017 |
| Title |
The histone acetyltransferase Mst2 prevents epigenetic silencing via specific acetylation of the ubiquitin ligase Brl1 [microarray data] |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Genome variation profiling by genome tiling array
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| Summary |
Faithful propagation of distinct chromatin states is crucial to maintain cellular identity. Whereas mechanisms that sustain repressed states have been intensely studied, regulatory circuits that protect active chromatin from inactivating signals are not well understood. Here we report the discovery of a self-reinforcing feedback loop that preserves the transcription-competent state of RNA polymerase II transcribed genes. We found that the chromatin reader Pdp3 recruits the histone acetyltransferase Mst2 to H3K36me3 marked chromatin. Thereby, Mst2 binds to all transcriptionally active regions genome-wide. Besides histone H3K14, Mst2 specifically acetylates Brl1, a component of the histone H2B ubiquitin ligase complex (HULC). Brl1 acetylation results in increased histone H2B ubiquitination, which together with H3K14ac positively feeds back on transcription and prevents assembly of ectopic heterochromatin. Our work uncovers a molecular pathway that secures epigenome integrity and highlights the importance of opposing feedback loops for the partitioning of chromatin into transcriptionally active and inactive states.
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| Overall design |
The study comprises 18 datasets from cells expressing a Dam fusion construct at low levels: 5 control Dam-only strains, 4 Dam-Mst2 in wild type cells, 5 Dam-Mst2 in pdp3D cells, 2 Dam-Mst2 in set2D cells, 2 Dam-Mst2 in set1D cells
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| Contributor(s) |
Flury V, Georgescu PR, Iesmantavicius V, Shimada Y, Kuzdere T, Braun S, Bühler M |
| Citation(s) |
28648780 |
| |
| Submission date |
Jan 11, 2017 |
| Last update date |
Sep 21, 2017 |
| Contact name |
Fabio Mohn |
| E-mail(s) |
fabio.mohn@fmi.ch
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| Organization name |
Friedrich Miescher Institute for Biomedical Research
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| Lab |
Buehler Lab
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| Street address |
Maulbeerstrasse 66
|
| City |
Basel |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL7715 |
[Sp20b_M] Affymetrix S. pombe Tiling 1.0FR Array |
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| Samples (18)
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| This SubSeries is part of SuperSeries: |
| GSE93434 |
The histone acetyltransferase Mst2 prevents epigenetic silencing via specific acetylation of the ubiquitin ligase Brl1 |
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| Relations |
| BioProject |
PRJNA361033 |
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