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| Status |
Public on Feb 22, 2017 |
| Title |
Gene expression data from WT and TET2cKO CD8+ T cells activated in vitro |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The epigenetic modifier TET2 plays a role in cell fate decisions in hematopeotic stem cells and CD4+ Th1 and Th17 differentiation. Here, we demonstrate that loss of TET2 promotes CD8+ memory differentiation following acute viral infection with LCMV-Armstrong.
To identify early gene expression changes following TCR activation in WT versus TET2cKO CD8+ T cells, we isolated naive CD8+ T cells and activated them for three days with anti-CD3/CD28+IL-2 and performed microarray analysis.
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| Overall design |
Naive (CD44lo CD62Lhi) CD8+ T cells were isolated and sorted from WT C57BL/6 and TET2fl/flCD4Cre+ mice. These cells were activated with plate-bound anti-CD3/CD28 for 3 days in the presence of 100U/ml hIL-2. RNA from these in vitro activated CD8+ T cells were processed, amplified, labeled and hybridized to Affymetrix GeneChip MoGene 2.0 ST microarrays.
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| Contributor(s) |
Carty SA, Koretzky GA, Jordan MS |
| Citation missing |
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| |
| Submission date |
Feb 21, 2017 |
| Last update date |
Feb 21, 2018 |
| Contact name |
Shannon Carty |
| E-mail(s) |
scarty@med.umich.edu
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| Organization name |
University of Michigan
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| Street address |
109 Zina Pitcher Place
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| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
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| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA376189 |
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