|
| Status |
Public on May 03, 2017 |
| Title |
The cohesin release factor WAPL restricts chromatin loop extension [DamID] |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
The spatial organization of chromosomes influences many nuclear processes including gene expression. The ring-shaped cohesin complex shapes the 3D genome by looping together convergent CTCF sites along chromosomes. We show here with high-resolution Hi-C analysis that chromatin loop size can be increased, and that cohesin’s DNA release factor WAPL restricts the degree of this extension. WAPL alsoincreased, and that cohesin’s DNA release factor WAPL restricts the degree of this extension. WAPL also prevents looping between incorrectly oriented CTCF sites. Through haploid genetics we find that WAPL deficiency bypasses the need for cohesin’s DNA loader SCC4 and we reveal that SCC4 promotes the extension of chromatin loops. We provide functional evidence in support of the model that chromatin loops are processively enlarged by the extrusion of DNA from cohesin rings. We conclude that the balanced activity of SCC4 and WAPL enables cohesin to correctly structure chromosomes to ensure proper transcriptional control.
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| |
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| Overall design |
DamID was performed as described (Peric-Hupkes et al., 2010).
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| |
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| Contributor(s) |
van der Weide RH, Yáñez-Cuna JO |
| Citation(s) |
28475897 |
| |
| Submission date |
Feb 28, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Robin H. van der Weide |
| Organization name |
Hubrecht Institute
|
| Lab |
Kind Lab
|
| Street address |
Uppsalalaan 8
|
| City |
Utrecht |
| State/province |
Utrecht |
| ZIP/Postal code |
3584 CT |
| Country |
Netherlands |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE95015 |
The cohesin release factor WAPL restricts chromatin loop extension. |
|
| Relations |
| BioProject |
PRJNA377319 |
| SRA |
SRP100866 |
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