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| Status |
Public on Nov 10, 2007 |
| Title |
Gene expression analysis of Peyers patches after infection of C57BL/6 mice with Yersinia enterocolitica |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Orogastral infection of mice with Yersinia enterocolitical leads to HIF-1 alpha activation.To elucidate whether this HIF-1 alpha activation also results in a HIF-1 dependent gene programming, the transcriptomes from Peyers Patches of uninfected and Yersinia enterocolitica infected mice were analyzed by means of of microarray analyses using Affymetrix GeneChip probe arrays (MG-U74Av2). In total, 288 genes were differentially regulated three day after infection in PP compared with the expression of uninfected control mice. Of these 288 genes, 217 were found to be differentially upregulated and from these, 14 genes ( 6.5% of all upregulated genes) are well described to be regulated via HIF-1. These data indicate that orogatral infection with Y. enterocolitica results in HIF-1 dependent gene programmning
Keywords: Time course
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| Overall design |
Per group five C57BL/6 mice were infected orogastrally with 500 Million Yersinia enterocolitica. 1 and 3 days after infection, Peyers Patches were removed and total RNA was prepared. In parallel, RNA was isolated from uninfected mice. The generation of fragmented cRNA was performed following the manufacturers instructions and used for hybridization onto GeneChip arrays MG-U74Avs2. Analysis of microarray data was performed using the Affymetrix Microarray Suite 5.0, Affymetrix Mining Tool 3.0. A median signal log2 ratio (SLR) grater than 1.5 or less than -1.5 was considered a significant change.
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| Contributor(s) |
Matteoli G, Hartmann H, Autenrieth IB, Kempf VA, Bohn E |
| Citation(s) |
18325389 |
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| Submission date |
Nov 08, 2007 |
| Last update date |
Feb 18, 2018 |
| Contact name |
Erwin Bohn |
| E-mail(s) |
erwin.bohn@med.uni-tuebingen.de
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| Phone |
00-49-70712981525
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| Fax |
00-49-7071294972
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| Organization name |
University of Tuebingen
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| Department |
Institutefor Medical Microbiology and Hygiene
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| Street address |
Elfriede-Aulhorn-Strasse 6
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| City |
Tuebingen |
| ZIP/Postal code |
72076 |
| Country |
Germany |
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| Platforms (1) |
| GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
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| Samples (3) |
| GSM241754 |
Gene expression in Peyers patches after orogatral Yersinia enterocolitica infection, Uninfected |
| GSM241770 |
Gene expression in Peyers patches after orogatral Yersinia enterocolitica infection, one day after infection |
| GSM241772 |
Gene expression in Peyers patches after orogatral Yersinia enterocolitica infection, three days after infection |
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| Relations |
| BioProject |
PRJNA103373 |
