|
| Status |
Public on Mar 08, 2017 |
| Title |
Platelet-derived growth factor receptor signaling is required for postnatal tendon growth |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Platelet-derived growth factor receptor (PDGFR) signaling plays an important role in the embryonic formation of many different tissues. There is a family of PDGF isoforms which signal through the PDGF receptors α (PDGFRα) and β (PDGFRβ). PDGF regulates many key cellular processes of mesenchymal cell function including proliferation, differentiation, migration and extracellular matrix (ECM) synthesis. While PDGF has been used to enhance flexor tendon healing in vivo, its role in postnatal tendon growth has remained largely unexplored. To determine the importance of PDGFR signaling in postnatal tendon growth, we performed pharmacological blockade of PDGFRα and PDGFRβ, and then induced tendon growth via mechanical overload using the hindlimb synergist ablation model. Our hypothesis was that inhibition of PDGFR signaling will restrict normal growth of tendon tissue in response to mechanical loading.
|
| |
|
| Overall design |
10 pooled samples total. Equal amounts of RNA isolated from four individual tendons were pooled into a single sample for microarray analysis, and two pooled samples from each group were analyzed. RNA was pooled because gene expression from a pooled sample is similar to the average of the individual samples composing the pooled sample.
|
| |
|
| Contributor(s) |
Sugg KB, Markworth JF, Disser NP, Rizzi AM, Talarek JR, Sarver DC, Mendias CL |
| Citation(s) |
29341790 |
| |
| Submission date |
Mar 07, 2017 |
| Last update date |
Mar 08, 2018 |
| Contact name |
Christopher Louis Mendias |
| E-mail(s) |
cmendias@umich.edu
|
| Phone |
734-764-4206
|
| Organization name |
University of Michigan
|
| Department |
Orthopaedic Surgery
|
| Lab |
Mendias Lab
|
| Street address |
109 Zina Pitcher Place
|
| City |
Ann Arbor |
| State/province |
Michigan |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17400 |
[MoGene-2_1-st] Affymetrix Mouse Gene 2.1 ST Array [transcript (gene) version] |
|
| Samples (10)
|
|
| Relations |
| BioProject |
PRJNA378415 |
Less...
More...